A simple, rapid and precise high-performance thin-layer chromatographic (HPTLC) method for analysis of candesartan cilexetil and hydrochlorothiazide in their tablet dosage forms has been developed and validated. Chromatography was performed on silica gel 60 GF
plates with acetone-chloroform-ethyl acetate-methanol 3:3:3:0.5 (
) as mobile phase. Detection was performed at 280 nm. The
values were 0.27 for candesartan cilexetil and 0.45 for hydrochlorothiazide. Regression plots revealed good linear relationships in the concentration ranges 50.6–253.0 μg for hydrochlorothiazide and 65.0–325.0 μg for candesartan cilexetil. When accuracy was checked by conducting recovery studies, average recovery was 99.78% and 98.09% for candesartan cilexetil and hydrochlorothiazide, respectively. The amounts of the drugs in the marketed formulation were 100.26%. and 99.28% for candesartan cilexetil and hydrochlorothiazide, respectively.
Authors:Bahia Moussa, Marwa Mohamed and Nadia Youssef
A simple, rapid, and selective densitometric thin-layer chromatographic (TLC) method has been established and validated for simultaneous quantitative analysis of olmesartan medoxomil and hydrochlorothiazide in the presence of olmesartan medoxomil degradation products. Chromatography was performed on aluminum foil-backed HPTLC plates coated with 0.2 mm layers of nano-silica gel 60 F254 as stationary phase. RF values of olmesartan medoxomil, its degradation products, and hydrochlorothiazide were significantly different when chloroform-methanol-formic acid 8:1.5:0.5 (v/v) was used as mobile phase. Detection was performed at 260 nm and 272 nm for olmesartan medoxomil and hydrochlorothiazide, respectively. Regression plots revealed good linear relationships in the concentration range 0.05–1 mg mL−1. Accuracy was checked by conducting recovery studies; average recovery was 100.35 ± 1.060 and 99.91 ± 1.154 for olmesartan medoxomil and hydrochlorothiazide, respectively. The amounts of the drugs in the dosage formulation were 102.78 ± 1.525% of the label claim for olmesartan medoxomil and 103.09 ± 1.259 for hydrochlorothiazide. Method validation was performed in accordance with USP guidelines.
Authors:Dorota Kowalczuk, Hanna Hopkała and Rafał Pietraś
A new, simple, and accurate TLC method, using normal- and reversed-phase techniques and densitometric detection, has been developed for measurement of quinapril and hydrochlorothiazide in combination tablets. UV detection at
= 210 nm was used to quantify the analytes. The drugs were chromatographed on silica gel 60 F
HPTLC plates and on octadecilsilane (RP-18) TLC plates, in horizontal chambers, with ethyl acetate-acetone-acetic acid, 8 + 2 + 0.5 (
) and methanol-0.07 m
phosphate buffer, pH 2.5, 6 + 4 (
), respectively, as mobile phases. The active substances were extracted from tablets with methanol (96% < mean recovery < 104%). Calibration curves were constructed in the range 0.4 to 2.4 μg μL
for quinapril and 0.25 to 1.5 μg μL
for hydrochlorothiazide, with good correlation (
≥ 0.998). The precision (
< 4.4%) and accuracy (2.91 <
< 3.92) were satisfactory for TLC-densitometric determination of quinapril in combination with hydrochlorothiazide in commercial tablets.
Authors:Anna Maślanka, Jan Krzek and Mariusz Stolarczyk
A new chromatographic and densitometric method has been established for identification and quantitative analysis of hydrochlorothiazide, triamterene, furosemide, and spironolactone, which are present, together, in complex drugs used to treat hypertension. For separation, silica gel F
plates were used with hexane-ethyl acetate-methanol-water-acetic acid 8.4:8:3:0.4:0.2 (v/v) as mobile phase. Densitometric measurements were performed at 264 nm selected for all of the constituents. The method is specific for the analyte constituents examined, and characterized by high sensitivity; LOD is from 0.022 to 0.150 μg per band, LOQ from 0.068 to 0.450 μg per band, recovery from 97.10 to 101.02%, and linear range from 0.060 to 2.650 μg per band. The method is characterized by good precision with RSD from 0.66 to 0.96%.
Authors:Hytham M. Ahmed, Tarek S. Belal, Rasha A. Shaalan, Fawzy A. El Yazbi and Sohila M. Elonsy
simultaneous analysis of benazepril hydrochloride (BEN) in 2 different binary combinations with amlodipine besylate (AML), and hydrochlorothiazide (HCT) in quality control laboratories (Figure 1 ).
Authors:A. B. Thomas, U. B. Chavan, R. K. Nanda, L. P. Kothapalli, S. N. Jagdale, S. B. Dighe and A. D. Deshpande
Simultaneous analysis of atenolol (Atn), hydrochlorothiazide (Hctz) and losartan potassium (Los) in solid dosage forms has been achieved by reversed-phase high-performance liquid chromatography on a C18 column with a 0.035 M potassium dihydrogen orthophosphate-acetonitrile gradient as mobile phase and UV detection at 225 nm. The retention times for Atn, Hctz, and Los were 2.91, 4.75, and 7.52 min, respectively, with mean recoveries of 99.67, 99.89, and 100.69%. The method was validated in accordance with ICH guidelines. Because of its simplicity and high precision and accuracy, the method can be used for analysis of atenolol, hydrochlorothiazide and losartan potassium in pharmaceutical preparations.