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-0124 Esfandani Bozchaloyi , S. , Sheidai , M. , Keshavarzi , M. and Noormohammadi , Z. ( 2017 d): Analysis of genetic diversity in Geranium robertianum by ISSR markers. – Phytologia Balcanica 23 ( 2 ): 157 – 166

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Three molecular markering techniques: inter-simple sequence repeat (ISSR); start codon targeted (SCoT), conserved DNA-derived polymorphism (CDDP) markers were compared for fingerprinting of 40 varieties of bread wheat. The number of scoreable and polymorphic bands produced using the ISSR, SCoT and CDDP primers for varieties was more than that of genotypes. Average polymorphism information content (PIC) for ISSR, SCoT and CDDP markers was 0.39, 0.41 and 0.34, respectively, and this revealed that three different marker types were equal for the assessment of diversity amongst genotypes. Cluster analysis for three different molecular types revealed that genotypes taken for the analysis can be divided in three and four distinct clusters. There were no significant differences among these markers in terms of the evaluation of genotypes. These results suggest that efficiency of SCoT, CDDP and ISSR markers was relatively the same in fingerprinting of genotypes but SCoT and CDDP analysis are more effective in fingerprinting of wheat genotypes. To our knowledge, this was the first detailed report of a comparison of performance among two targeted DNA region molecular markers (SCoT and CDDP) in comparision with ISSR technique on a set of samples of wheat cultivars. Overall, our results indicate that SCoT, ISSR and CDDP fingerprinting could be used to detect polymorphism for genotypes of wheat.

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Paczos-Grzęda, E. 2007. Wykorzystanie metod ISSR i RAPD oraz analizy rodowodów do oceny podobieństwa międzyodmianowego Avena sativa (Cultivars similarity estimation based on RAPD and ISSR methods and pedigree analysis). Zesz. Probl. Post. Nauk Rol. 517

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Bornet, B., Branchard, M. 2001. Nonanchored Inter Simple Sequence Repeat (ISSR) markers: Reproducible and specific tools for genome fingerprinting. Plant Mol. Biol. Rep. 19 :209–215. Branchard M

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. , Zargar , O. , Sharma , V. and Gupta , R. Ch. ( 2016 ): Genetic diversity and population structure of Rheum species in Kashmir Himalaya based on ISSR markers . – Flora 223 : 121 – 128 . https://doi.org/10.1016/j.flora.2016

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by ISSR markers . – Phytol. Balcan. 23 ( 2 ): 157 – 166 . Esfandani-Bozchaloyi , S. , Sheidai , M. , Keshavarzi , M. and Noormohammadi , Z. ( 2018 a): Species relationship

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ISSR molecular markers . — Acta Bot. Croat. 75 : 45 – 52 . https://doi.org/10.1515/botcro-2016-0005 Schönbeck-Temesy , E. ( 1970 ): Geraniaceae . — In: Rechinger , K

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Nymphaea species, the most popular decorative plants, were collected for specificity of inter-simple sequence repeat (ISSR) analyses in species identification and differentiation of cultivars and natural populations. Dendrogram constructed from ISSR analyses separated out wild species, namely Nymphaea cyanea, N. nouchali, N. capensis, N. lotus and an outgroup N. mexicana, and cultivars. The dendrogram indicates that the cultivars should be differentiated from N. capensis, as they are sister individuals of N. capensis. The ISSR banding data and the dendrogram are concordantly concluded that wild N. capensis would be an effective type species for producing different cultivars. After plant identification by ISSR markers, DNA barcodes of all sample materials were done to provide species specific markers which can be used for rapid and accurate further plant identification without morphological characters. DNA barcoding sequence analysis indicates genetic distance values. All sequences were recorded in GenBank database.

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DNA methylation polymorphism among nine elite maize inbred lines was assessed by inter-simple sequence repeat (ISSR) fingerprinting on intact DNA and DNA digested by either of a pair of methylation-sensitive isoschizomers, Hpa II and Msp I. It was found that, along with distinct genetic differentiation, extensive DNA methylation polymorphism exists among the nine inbred lines. The line-specific methylation patterns are homogeneous within each line, indicating their usefulness as molecular markers for cultivar identification. DNA sequences underlying the DNA methylation variations include a high proportion of coding genes. Cluster analysis, however, indicates the existence of incongruence between DNA methylation polymorphism and known-pedigree of the maize inbred lines.

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. Baysal , Ö. , Siragusa , M. , Gümrükcü , E. , Zengin , S. , Carimi , F. , Sajeva , M. , Teixeira da Silva , J.A. : Molecular characterization of Fusarium oxysporum f. melongenae by ISSR and RAPD markers on eggplant . Biochem Genet 48

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