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using ultra-high performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS). In the present study, we established a simple, sensitive, and rapid liquid chromatography (LC)–MS/MS method and validated for determination of PH in dog plasma
–tandem mass spectrometry (LC–MS/MS) method has been developed and validated for simultaneous determination of PA-824, moxifloxacin, and pyrazinamide in rat plasma and its application to a pharmacokinetic study in rats. To further clarify the DDI between the
ng/mL and an elimination half-life of about 8 h [ 4 ]. Literature survey revealed that LCZ has been reported to be determined by ultraviolet (UV) spectrophotometry [ 3 , 5 ], liquid chromatography–tandem mass spectrometry (LC–MS/MS) in human plasma
In the present study, the degradation behavior of Fenofibrate under different International Conference on Harmonization (ICH) suggested conditions was studied. Characterization of degradation products by liquid chromatography–tandem mass spectrometry (LC–MS/MS) studies in solution form was done, and the possible mechanism for the formation of degradants is discussed. Fenofibrate was subjected to different hydrolytic stress conditions and thermal stress condition (in solid form). Successful separation of drug from degradants was achieved on a C18 column using water–acetonitrile (25:75 v/v) as the mobile phase. Other high-performance liquid chromatography (HPLC) parameters were: flow rate, 1 mL min−1; detection wavelength, 286 nm; column temperature, 25 °C; and injection volume, 20 μL. The method was validated for linearity, precision, accuracy, robustness, and specificity and was stability-indicating one, based on the specificity studies. The drug degraded under acidic, basic, and oxidative hydrolytic stress while it was relatively stable towards neutral hydrolysis and thermal stress. The stressed samples were subjected to LC–MS/MS analysis. On the basis of spectral data, the structures of four degradation products and one interaction product were suggested. Degradation products were characterized to be isopropyl acetate, 2-[4-(4-chlorobenzoyl)phenoxy]-2-methyl propanoic acid, 4-hydroxy benzoic acid, and benzoic acid. The structure of one interaction product was proposed as methyl 2-[4-(4-chlorobenzoyl)phenoxy]-2-methylpropanoate.
shortening of drug-susceptible and XDR-TB [ 16 ]. In the current work, we conducted studies to characterize the potential for a pharmacokinetic interaction between pretomanid and pyrazinamide in rats using LC-MS/MS. We have developed and validated an LC-MS/MS
[ 10 ]. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is one of the most sensitive analytical methods for the detection, identification and quantification of analyzed compounds on biological samples. Literature survey reveals that
chromatography-tandem mass spectrometry (LC-MS/MS) method were developed, respectively [ 9 , 11 ]. However, there is little bioanalytical method for the determinatin of solasodine in urinary and fecal samples. In addition, bioanalysis of urine is commonly
Method Internal standard Matrix Matrix volume (μL) Sample preparation Elution Column Mobile phase Linearity range (ng mL −1 ) Analytical time (min) Reference 2015 LC-MS/MS ER-167615 Plasma 100 LLE by MTBE Isocratic YMC-Pack Pro C8 column (50 × 3.0 mm) 0
determination of PG can be found in the literature. Liquid chromatography coupled with a tandem mass spectrometer (LC-MS/MS) is preferable method due to its selectivity, accuracy, precision, and robustness [ 13–15 ]. Some of the published methods require a
LC–MS/MS method for simultaneous monitoring of RIV and SIT and applied it to the pharmacokinetic study in rats to evaluate DDI interaction between the two drugs in rats. Experimental Chemicals and
