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shortening of drug-susceptible and XDR-TB [ 16 ]. In the current work, we conducted studies to characterize the potential for a pharmacokinetic interaction between pretomanid and pyrazinamide in rats using LC-MS/MS. We have developed and validated an LC-MS/MS

Open access
Acta Chromatographica
Authors:
Jelena Dzudovic
,
Milkica Crevar Sakac
,
Marko Antunovic
,
Aleksandra Repic
,
Slobodan Obradovic
,
Snezana Djordjevic
,
Jelena Savic
, and
Boris Dzudovic

[ 10 ]. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is one of the most sensitive analytical methods for the detection, identification and quantification of analyzed compounds on biological samples. Literature survey reveals that

Open access
Acta Chromatographica
Authors:
Tiantian Lu
,
Xiaohong Wang
,
Qi Zhang
,
Kun Liu
,
Tongxin Xu
,
Quande Wang
,
Pengfei Zhao
, and
Zhongzhe Cheng

chromatography-tandem mass spectrometry (LC-MS/MS) method were developed, respectively [ 9 , 11 ]. However, there is little bioanalytical method for the determinatin of solasodine in urinary and fecal samples. In addition, bioanalysis of urine is commonly

Open access
Acta Chromatographica
Authors:
Hao-ran Dai
,
Ya-hui Hu
,
Jia-yi Long
,
Ying Xia
,
Hong-li Guo
,
Jing Xu
,
Xuan-sheng Ding
,
Jing Chen
,
Xiao-peng Lu
, and
Feng Chen

Method Internal standard Matrix Matrix volume (μL) Sample preparation Elution Column Mobile phase Linearity range (ng mL −1 ) Analytical time (min) Reference 2015 LC-MS/MS ER-167615 Plasma 100 LLE by MTBE Isocratic YMC-Pack Pro C8 column (50 × 3.0 mm) 0

Open access

LC–MS/MS method for simultaneous monitoring of RIV and SIT and applied it to the pharmacokinetic study in rats to evaluate DDI interaction between the two drugs in rats. Experimental Chemicals and

Open access

% of the original drug are excreted through the kidney [ 7 ]. HPLC-UV [ 8 , 9 ], HPLC-FL [ 10 ], Micellar electrokinetic chromatography [ 11 , 12 ] and LC-MS/MS [ 13–16 ] method has been developed for determination of letrozole in plasma

Open access

green analytical method to improve the test method of cordycepin and isocordycepin in C. sinensis and other Cordyceps samples. Liquid chromatography tandem mass (LC-MS/MS), which can provide high selectivity and short analysis time, has been employed

Open access
Acta Chromatographica
Authors:
L. S. Teixeira
,
I. M. Mundim
,
W. C. Souza
,
D. R. Ramos
,
K. B. Bellorio
, and
K. R. Rezende

Summary

A simple, sensitive, and rapid liquid chromatography-mass detection (LC-MS/MS) method for the analysis of betamethasone (BET) from intramuscular injection of phosphate and dipropionate BET produgs was developed for bioequivalence studies in human plasma. The calibration curve was linear over the range of concentrations (0.5–50.0 ng mL−1; r 2 = 0.99), showing a very high sensitivity without interferences at the retention times of BET (0.8 min) and the internal standard (IS) triamcinolone acetonide (0.95 min). Both drug (D) and IS were extracted from human plasma by liquid-liquid extraction, showing average recovery values of 94.0 and 98.9%, respectively. Within- and between-run precision studies demonstrated a variation coefficient <10% at all tested concentrations. Therefore, our analytical method proved to be validated according to the worldwide-accepted FDA guidelines and successfully applied for bioequivalence studies of parenteral formulations containing BET dipropionate (5 mg mL−1) and BET sodium phosphate (2 mg mL−1).

Open access

spectrometry (LC-MS/MS) method [ 11 , 12 , 15 ]. Compared with HPLC method, LC-MS/MS method is more sensitive and shows high resolution, and can significantly reduce analysis time and increase sample throughput. However, as for those reported LC-MS/MS methods

Open access

A new liquid chromatography-tandem mass spectrometric (LC-MS/MS) method was developed to confirm chloramphenicol (CAP) residues in foods of animal origin and in urine samples, which were earlier found positive under the screening analysis, performed by competitive enzyme-linked immunoassay (ELISA) technique. The developed LC-MS/MS method was applied to four non-compliant samples from 2008 to 2012; giving concentrations of CAP residues from 1.18 to 3.68 μg kg−1. All samples, qualified positive by ELISA, were confirmed with the LC-MS/MS technique and found to be non-compliant. The effectiveness of the confirmatory method was proven by participating in a successful proficiency test in year 2010. Both LC-MS/MS and ELISA methods were validated according to the European Union 2002/657/EC decision. The decision limit of the confirmatory method was determined as 0.02 μg kg−1 for CAP in each validated matrix, while the detection capability of the screening test was 0.15 μg kg−1.

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