identification ( Böhme et al., 2011 ; Alnakip et al., 2020 ). In recent years, MALDI-TOFMS (matrix assisted laser desorption ionisation-time of flight mass spectrometry) has become a popular technique in microbiological identification. The application of MALDI-TOF
Introduction The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) provides a fast, simple and cost-effective diagnosis in clinical microbiology laboratories, which is increasingly found a place in the
uropathogens in urine by MALDI-TOFMS can significantly shorten the identification time from 24 to 48 h, using classical methods, to 30 min [ 7, 8 ]. In our study, our aim was to detect positive urine samples by flow cytometry and to identify bacteria directly
molecular biology techniques (Agius et al., 2007). Matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI- TOFMS) allows the identification of microorganisms isolated from food or clinical cases by a low-cost, rapid, easy
Lipids are important natural products and essential in nutrition, cosmetic formulations, pharmaceuticals, etc. Lipids and, particularly, phospholipids are of substantial medical interest (some are molecules with messenger function) and of diagnostic potential (for instance, the lipoproteins in human blood). Among the different soft-ionization mass spectrometric methods that enable detection of the intact lipid molecules, matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has several advantages, for instance, simple performance, high sensitivity, and robustness against contaminants. Additionally, MALDI-TOF MS analyzes a solid sample. This enables (in contrast with isotropic solutions) acquisition of spatially-resolved mass spectra (‘mass spectrometric imaging’). However, separation of complex mixtures into the individual lipid classes is normally required to enable detection of all the components. It will be shown with the example of a lipid extract from hens’ egg yolk that MALDI-TOF MS can be easily combined with TLC, enabling detection of as little as picomole amounts of lipids directly on the HPTLC plate. This results in sensitivities higher than those from established staining procedures. Additionally, because of the substantial spatial resolution, lipids separated by normal-phase TLC may not only be differentiated according to differences of their headgroups but also according to differences of their fatty acyl composition. Finally, MS-MS experiments, providing further insights into the structures of the relevant lipids, can be also performed directly on the HPTLC plate. Although the HPTLC-MALDI coupling can be easily established, there are different points to which special attention should be paid. Aspects of matrix application, data acquisition (including the stability of lipids and reproducibility), and data evaluation will be emphasized in this paper
Members of the genus Bacteroides are important components of the normal microbiota of gastrointestinal tract; however, as opportunistic pathogens are also associated with severe or even life-threatening infections with significant mortality. Various species within Bacteroides fragilis group are phenotypically very similar; thus, their identifications with traditional-automated biochemical methods are frequently inaccurate. The identification of the newly discovered or reclassified bacteria can be doubtful because of the lack of biochemical profile in the database of these tests. The aim of this study was to determine the accuracy of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method by testing of 400 Hungarian Bacteroides clinical isolates. Inaccurate identification results with MALDI-TOF MS were confirmed by 16S rRNA gene sequencing and findings were compared with traditional-automated biochemical test rapid ID 32A method as well.
We report the case of a nosocomial infection due to Enterococcus cecorum isolated from a blood culture of a 75-year-old septic male patient. Matrix-assisted laser desorption–ionization time-of-flight mass spectrometry (MALDI-TOF MS) and Vitek 2 succeeded in identification of the isolate.
, 2009; Becker et al., 2014 ). Similar to the emergence of polymerase chain reaction, the backbone of modern molecular biology, matrix-assisted laser desorption ionisation–time of flight mass spectrometry (MALDI-TOFMS) has also become a paradigm
Laser desorption ionization (LDI) mode of matrix-assisted laser desorptionionization time-of-flight mass spectrometry (MALDI-TOFMS) analysis of uranium(VI)leads to the formation of uranium oxides clusters, as with fast atom bombardment(FAB). Different uranium clusters than those with FAB were observed. Threedifferent families of formula (UO2)x Oy2+, and two of formula (UO2)x Oy2+ were found.
appropriate study conditions (number 2) and followed the steps established to USP 38, the main guide for verification of microbial identification methods. Thus, the objective of this work was to evaluate the suitability of the MALDI-TOFMS technique for the