Authors:Gyöngyvér Gell, Kathrin Petrik and E. Balázs
During our recent four years epidemiological studies ofMaize dwarf mosaic virus (MDMV) populations in Hungary, when 86 virus isolates were collected and analysed, a unique nucleic acid sequence variant was found in the 3’end region of the viral genome. According to our sequence studies, which also included all available MDMV sequences from different databanks, the coat protein region of the viral genome prooved to be quite identical. However, in several cases an insertion was located in the same position of the coat protein region. In the unique sequence variant we also found a 27 nt deletion in addition to the insertion. According to the extensive sequence search this deletion is unique and have been located only in two other potyvirus coat protein regions, while never in the case of MDMV. The potential role of this deletion in the virus infectivity, replication or other biological characteristics is discussed.
Authors:I. Tóbiás, N. Bakardjieva and L. Palkovics
Maize dwarf mosaic is the most widespread virus disease affecting corn production in Hungary and Bulgaria. Samples from virus infected maize were collected from different part of Bulgaria and employed test plants, ELISA serological method and RT-PCR in order to identify the viral pathogen.
Maize dwarf mosaic virus
(MDMV) was detected in all tested samples. For further investigation three MDMV isolates were selected and cloned. Cloned cDNAs representing the coat protein gene of the virus have been sequenced. The coat protein genes of Bulgarian and Hungarian isolates of MDMV were compared. The nucleotide sequence identity and amino acid sequence similarity of the coat protein region varied from 88% to 99.1% and from 95.1% to 99.6%, respectively. The N-terminal region of coat protein was compared with other members SCMV subgroup and phylogenetic tree was constructed.
, E. Fuchs, M. Gruntzig, B. Schulz, D. Klein, A.E. Melchinger
(1997): Resistance of early-maturing European maize germplasm to sugarcane mosaic virus (SCMV) and maize dwarf mosaic virus (MDMV). — Plant Breeding