Search Results

You are looking at 1 - 10 of 229 items for :

Clear All

pathogenic bacteria surviving in the intestine, depending on the sensitivity to glyphosate. Regarding a ruminal setting, these findings could not be confirmed [ 19 ]. Shehata et al. [ 20 ] determined differing minimum inhibitory concentrations (MIC) for

Open access
Acta Microbiologica et Immunologica Hungarica
Authors: Seyedeh Marzieh Jabbari Shiadeh, Leila Azimi, Taher Azimi, Ali Pourmohammad, Mehdi Goudarzi, Bahare Gholami Chaboki and Ali Hashemi

Concentrations (MICs) of different antimicrobials and the expression of efrAB genes in ciprofloxacin resistant E.faecalis strains by quantitative Real-Time PCR (qRT-PCR). Finally, we chose more resistant isolates for typing by Multilocus Sequence Typing (MLST

Restricted access

: Reconstituirea evoluiei geomorfologice a Văii Someşul Mic în Holocen [Reconstruction of the geomorphological evolution of the Someşul Mic Valley in the Holocene]. PhD manuscript. Iaşi 2010. Feier I

Restricted access

(30 μg) (Oxoid, USA). Minimum inhibitory concentration (MIC) values were determined by gradient test for vancomycin and teicoplanin (bioMérieux, France) ( Table 1 ). The Enterococcus strains were considered resistant if the MIC values of vancomycin

Restricted access

(30 μg) (Oxoid, USA). Minimum inhibitory concentration (MIC) values were determined by gradient test for vancomycin and teicoplanin (bioMérieux, France) ( Table 1 ). The Enterococcus strains were considered resistant if the MIC values of vancomycin

Restricted access

In vitro antimicrobial sensitivity of 12 Hungarian isolates and the type strain ATCC 33144 of Actinobaculum suis to different antimicrobial compounds was determined both by the agar dilution and by the disc diffusion method. By agar dilution, MIC50 values in the range of 0.05-3.125µg/ml were determined for penicillin, ampicillin, ceftiofur, doxycycline, tylosin, pleuromutilins, chloramphenicol, florfenicol, enrofloxacin and lincomycin. The MIC50 value of oxytetracycline and spectinomycin was 6.25 and 12.5µg/ml, respectively. For ofloxacin, flumequine, neomycin, streptomycin, gentamicin, nalidixic acid, nitrofurantoin and sulphamethoxazole + trimethoprim MIC50 values were in the range of 25-100µg/ml. With the disc diffusion method, all strains were sensitive to penicillin, cephalosporins examined, chloramphenicol and florfenicol, tetracyclines examined, pleuromutilins, lincomycin and tylosin. Variable sensitivity was observed for fluoroquinolones (flumequine, enrofloxacin, ofloxacin), most of the strains were susceptible to marbofloxacin. Almost all strains were resistant to aminoglycosides but most of them were sensitive to spectinomycin. A strong correlation was determined for disc diffusion and MIC results (Spearman's rho 0.789, p<0001). MIC values of the type strain and MIC50 values of other tested strains did not differ significantly. Few strains showed a partially distinct resistance pattern for erythromycin, lincomycin and ampicillin in both methods.

Restricted access

was tested to 18 antimicrobial agents using minimal inhibitory concentration (MIC) test strips according to the manufacturer's instructions (Liofilchem, Roseto, Italy; Table 2 ). Escherichia coli ATCC 25922 served as a quality control strain. MIC

Open access

was tested to 18 antimicrobial agents using minimal inhibitory concentration (MIC) test strips according to the manufacturer's instructions (Liofilchem, Roseto, Italy; Table 2 ). Escherichia coli ATCC 25922 served as a quality control strain. MIC

Open access

The presence of the vanA gene was determined in enterococci from healthy poultry, originating from the Hungarian resistance monitoring system between 2001 and 2004. Enterococci (n = 562) were collected from intestinal samples of slaughtered broiler chickens. The presence of van genes was detected by polymerase chain reaction (PCR). The vancomycin-resistant enterococcus (VRE) strains carried only the vanA gene. Genus- and species-level identification of the vanA gene carrier strains was carried out by PCR using specific primers. In 2001, 25 out of the 289 isolated strains (8.6%) were vanA carriers (1 Enterococcus mundtii , 13 E. durans and 11 E. faecium ). In 2002 (n = 87), 20 (23%) strains were vanA positive (11 E. durans and 9 E. faecium ). In 2003 and 2004, none of the strains (n = 95 and 91, respectively) were positive for the most common van genes. In 2003, there was only one strain for which higher minimum inhibitory concentrations (MIC) of vancomycin (4 mg/L) and teicoplanin (8 mg/L) were found. In 2004 there were three strains for which the MIC of vancomycin was 8 mg/L, and 2 strains and 1 strain with teicoplanin MICs of 4 mg/L and 8 mg/L, respectively. The potential similarity of these strains was studied by pulsed-field gel electrophoresis (PFGE). The VRE strains were not closely related to one another. The annual data of vancomycin resistance indicate an association between the recovery of vancomycin-resistant enterococci and the use of avoparcin in animal feeds. This study indicates that with the reduced use of antibiotics in food animals, it is possible to decrease the rate of resistant bacteria. Although the use of avoparcin had been banned in 1998, the VRE strains disappeared only five years later.

Restricted access

performed in accordance with the recommendations of National Centre for Susceptibility Testing [ 16 ]. Phenotypic detection of colistin resistance Minimum inhibitory concentration (MIC) of colistin was determined by

Restricted access