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HPTLC analysis has been used to compare neutral lipid profiles in the urine of humans and mice. Neutral lipids and ubiquinone were extracted from urine with chloroform-methanol, 2:1, and determined on silica gel plates with 19 lanes and a concentration zone that were developed with petroleum ether-diethyl ether-glacial acetic acid, 80:20:1. Separated zones were detected with phosphomolybdic acid reagent and quantified by visible mode slit-scanning densitometry at 610 nm. Specific detection reagents were used to confirm the identity of particular lipid classes. The studies confirmed the presence of free sterols, free fatty acids, and triacylglycerols in both human and mouse urine. Methyl esters were found in mouse but not human urine. Hydrocarbons and ubiquinone were present in both human and mouse urine, but were not quantified. Similarities in the urinary neutral lipid profiles of humans and mice suggest that mice may serve as effective models for studies of the effects of infectious and metabolic diseases in humans.

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The purpose of this study was to identify and quantify various neutral and polar lipids in certain organs of mice using high-performance thin-layer chromatography (HPTLC). Four mice infected with Schistosoma mansoni and three control mice were used for this study. At 6 weeks postinfection, the mice were necropsied, and the liver, spleen, and small intestine were removed and prepared for lipid analysis. Lipids were separated on laned, preadsorbant Analtech HPTLC-HLF 20 × 10-cm silica gel plates. Neutral lipids were separated using petroleum ether-diethyl ether-glacial acetic acid (80:20:1) mobile phase and were detected by spraying with 5% ethanolic phosphomolybdic acid detection reagent. Polar lipids were separated with chloroform-methanol-deionized water (65:25:4) mobile phase and detected using 10% cupric sulfate in 8% phosphoric acid reagent. The analyzed neutral lipids were free sterols, free fatty acids, and triacylglycerols. Using HPTLC, no significant differences were found in these lipids between the infected and uninfected mice organs. The polar lipids analyzed were phosphatidylcholine (PC) and phospatidylethanolamine (PE). There was a significantly higher PC content in the liver and small intestine of the uninfected mice compared with that of infected mice.

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High-performance thin-layer chromatography was used to examine the phospholipid profiles in the urine of Echinostoma caproni infected mice, uninfected mice, and humans. Phospholipids were extracted from urine with chloroform-methanol, 2:1, and determined on silica gel plates with 19 lanes and a concentration zone that were developed with chloroform-methanol-water, 65:25:4. Separated zones were detected by charring with aqueous cupric sulfate reagent and quantified by visible mode slit-scanning densitometry at 370 nm. Ninhydrin spray reagent was used to confirm the presence of phosphatidylethanolamine and spiking analyses were used to confirm the identity of phosphatidylcholine in human urine. Lysophosphatidylcholine was found in human but not mouse urine. Comparison of chromatograms from the urine of infected and uninfected mice showed no qualitative or quantitative differences in the phospholipid profiles, suggesting that urinary phospholipids may not serve as biological markers for trematode infection in mice. Marked differences between the overall polar lipid profiles of mouse and human urine suggest that mice may not be useful models for humans in analyses of the effects of metabolic and infectious diseases on polar lipids.

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. Horiguchi , Y. , Matsuda , H. , Koyama , H. , Nakai , T. and Kume , K. ( 1992 ): Bordetella bronchiseptica dermonecrotizing toxin suppresses in vivo antibody responses in mice . FEMS Microbiol. Lett. 69 , 229 – 234

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Acta Biologica Hungarica
Authors: V. Posevitz, C. Vizler, S. Benyhe, E. Duda, and Anna Borsodi

. 121 384 390 Palanza, P., Gioiosa, L., Parmigiani, S. (2001) Social stress in mice: gender differences and effects of estrous cycle and social

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this study, co-culture of SGNs and hair cells and antibody blocking are utilized to investigate the function of Cx43 during the early development and adult period of mice cochlea. Materials and methods Ethics statement C57BL/6J mice were ordered from

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, M., Stevenson, W., Choi, J., Latimer, K. S., Kanitz, C. L. and Mittal, S. K. (2001): Viral replication and lesions in BALB/c mice experimentally inoculated with porcine circovirus isolated from a pig with postweaning multisystemic wasting disease

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Acta Veterinaria Hungarica
Authors: Zhi Deng, An Yuan, Wei Luo, Nai Wang, Qian Gong, Xing Yu, and Li Xue

Cságola, A., Cadar, D. and Tuboly, T. (2008): Replication and transmission of porcine circovirus type 2 in mice. Acta Vet. Hung. 56 , 421–427. Tuboly T. Replication and transmission of

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Acta Physiologica Hungarica
Authors: A. Moslehi, Fatemeh Nabavizadeh, A.R. Dehpou, S.M. Tavanga, G. Hassanzadeh, A. Zekri, H. Nahrevanian, and H. Sohanaki

Amin A, Choi SK, Galan M: Chronic inhibition of endoplasmic reticulum stress and inflammation prevents ischaemia-induced vascular pathology in type II diabetic mice. J Pathol. 227, 165–174 (2012) Galan M

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model with alloxan, I found that Moringa promotes the activity of both CD4 + and CD8 + T cells in diabetic treated mice that may occur through the Sca-1 + CD117 + stem cell factors, which play an important role as hematopoietic regulators (data not

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