Authors:Ádám Dávid, Emil Mincsovics, István Antal, Éva Furdyga, Zsolt Zsigmond, and Imre Klebovich
We report a new combination of overpressured-layer chromatography (OPLC) with near-infrared (NIR) spectroscopy for pharmaceutical analysis. Different pharmaceutical preparations containing caffeine, paracetamol, and acetylsalicylic acid as model compounds were separated by OPLC. The band density in the solid phase after OPLC was suitable for study of the separated components directly on the layer by NIR spectroscopy.We have demonstrated the applicability of rapid OPLC separation combined with UV densitometry and NIR spectroscopy for both qualitative and quantitative analysis. This OPLC-UV-NIR technique is thus suitable for rapid, nondestructive investigation of multicomponent pharmaceutical preparations and enables a different type of pharmaceutical analysis, e.g. starting-material tests, in-process control, end-product control, stability testing, etc. Another benefit of this newly developed combination of rapid off-line techniques is the possibility of simultaneous collection of qualitative and quantitative chromatographic and spectral information.
Authors:Zsanett Bodor, Fanni Adrienn Koncz, Mahmoud Said Rashed, Timea Kaszab, Zoltan Gillay, Csilla Benedek, and Zoltan Kovacs
, A. , Slabbert , R. , & Manley , M. ( 2017 ). Verification of authenticity of South African honey and fraud detection using NIRspectroscopy . Food Control , 73 , 1388 – 1396 . doi: http://dx.doi.org/10.1016/j.foodcont.2016
Authors:E. Nagy, I. Timár, Z. Hegyi, T. Spitkó, and L. Marton
The chemical composition of maize makes it suitable for a wide range of nonconventional uses, including utilisation as a new source of energy for the 21st century as a raw material for biofuel. The aim of the experiments was to amalgamate the application of genetic markers with conventional breeding methods to produce maize hybrids whose starch content and ecostability satisfied the demands of industrial use, while having yield potential and agronomic traits on a par with those of hybrids currently cultivated. The chemical quality of 220 lines was evaluated using the NIR spectrometric technique, and the five maize inbred lines with the lowest and highest starch contents were selected for genetic marker studies. The variety identification of the lines was carried out using the isoenzymes stipulated by UPOV. The following SSR (simple sequences repeat) markers were tested: phi 095, umc 1057, nc 004, phi 096, nc 007, umc 1564, phi 85, y1 SSR, umc 1178, nc 009, phi 070, umc 1066, umc 1741, umc 1069, phi 033, phi 061, wx, phi 032, phi 084 and phi 062. The analysis of the fragment patterns revealed three SSR markers that appeared to be correlated with the starch content of the maize lines. These were the primer pairs
y1 SSR, umc 1069
. These results are only of a preliminary nature, however, as the incorporation of starch is probably regulated by several genes, and the studies suggest it is also influenced by several environmental factors. It also appears likely that the bioethanol yield is determined not only by the starch content, but also by other parameters. Further research should thus be expanded to include investigations into the structural and fermentability traits of starch molecules, including the characterisation of these traits using genetic markers.
Authors:K. Horváth, Zs. Seregély, É. Andrássy, I. Dalmadi, and J. Farkas
Experimental batches of chilled boneless slices of pork meat have been stored aerobically in sterile Petri dishes and total aerobic plate counts (TAPC) and sensorial observations were made periodically during storage to monitor bacterial growth and apparent deteriorative changes at 4, 8 and 12 °C, respectively. Near infrared spectroscopy (diffuse reflectance) measurement was performed on replicate meat samples in the wavelength range of 1000–1800 nm. Second derivative and multiplicative scatter correction were performed on the spectra as data pre-treatments. Principal component analysis (PCA) and canonical discriminant analysis (CDA) were used for observation of discrimination of the samples due to loss of freshness and onset of bacterial spoilage as a function of the storage time. The percentage of correctly classified samples decreased somewhat by increasing the storage temperature. Partial least squares (PLS) chemometric model was developed to predict and quantify bacterial loads from the scatter corrected 2nd derivative spectra. PLS evaluation (predicted versus measured TAPC values) — when bacterial counts at all sampling days and storage temperatures were taken into account — resulted in a correlation coefficient of 0.977, and a root mean square error of prediction (RMSEP) 0.438 log colony forming units/g. These preliminary results indicate the potential of utilising near infrared diffuse reflectance spectroscopy in combination with multivariate statistical methods to monitor loss of freshness and detect bacterial spoilage of meat samples rapidly before deleterious microbial changes become apparent. However, much larger number of samples should be studied to ascertain properly the prediction power of the spectroscopic method.
Authors:M. Bernardi, E. Leite, E. Longo, M. Conceiçăo, and A. Souza
The color efficiency of ceramic glaze blue pigments obtained by the Pechini method was presented in this work. The fired pigments
and enameled samples were characterized by thermogravimetry (TG) and differential thermal analysis (DTA), XRD, UV-VIS-NIR
spectroscopy and CIE-L*a*b* color-measurements. The pigments obtained by the Pechini method present a better solubility in
the molten glazes than the pigments obtained by the mechanical mixture of the precursor oxides.
Kaffka, K. J. (1992): Qualitative analysis applying NIRspectroscopy. Taylor, R. A. (Ed.) Proceedings: International Diffuse Reflectance Spectroscopy Conferences , The Council for Near Infrared Spectroscopy
Authors:S. Janarthanan, R. Sugaraj Samuel, Y. C. Rajan, and S. Pandi
Semicarbazone of dl-camphor (SdlC) crystals were grown using methanol as a solvent by slow evaporation solution growth technique at room temperature. Formation of the product and the presence of various functional groups present in the grown crystal have been identified using FTIR spectra. Single crystal XRD study was conducted to obtain the crystal structure and lattice parameters. The grown crystal was subjected to 1HNMR and 13CNMR spectral studies and TG-DTA in order to confirm its structure, purity, and stability, respectively. The optical transparency of the crystal was tested using UV–Vis–NIR spectroscopy. The nonlinear optical (NLO) property of the grown crystal was confirmed from the second harmonic generation (SHG) by Kurtz–Perry powder test.
Authors:L. Locsmándi, G. Kövér, G. Bázár, A. Szabó, and R. Romvári
The feasibility of NIR spectroscopy for
determining chemical composition of goose fatty liver was studied. The spectra
of 50 fresh, homogenized liver samples (ether extract content EE=53.2,
SD=4.87%) were taken between 1100 and 2500 nm in reflectance mode, then the
chemical composition and the fatty acid profile was measured (gas
chromatography). Calibration equations were developed using modified partial
least-squares regression. The R
value in estimation of DM, CP and total EE were 0.72, 0.63 and 0.81,
respectively. For the major fatty acids (oleic, palmitic and stearic acid,
51.4, 25.8 and 15.5% of total) the R² values were 0.94, 0.93 and 0.16. The
estimation of the total saturated fatty acid (SAT) proportion and the so-called
unsaturation index (UI) value was effective (R
=0.81 and 0.79, respectively).
The paper demonstrates the usefulness of the NIRS method as a fast and solvent
free alternative of liver qualification. For practical purposes a larger number
of fatty liver samples are needed.