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Abstract  

Differential scanning microcalorimetry (DSC) and UV–VIS absorption spectroscopy were used to obtain the characteristics of blood serum from newborn rat’ after maternal treatment with cyclophosphamide in comparison with control. The obtained DSC curves reveal a complex endothermic peak due to the unfolding process of various serum proteins. Thermal profiles and absorption spectra of blood serum are sensitive to the age of newborns as well as to effect of maternal administration of cyclophosphamide. The most significant disturbances in serum proteome were observed for 14-day old newborns. The thermodynamic parameters: enthalpy change (∆H), the normalized first moment (M1) of the thermal transition with respect to the temperature axis and the ratio of C p ex at 70 and 60 °C describing denaturation contributions of globulin forms in respect to unliganded albumin with haptoglobin was estimated. Moreover, the second derivative spectroscopy in the UV region was used to resolve the complex protein spectrum. The differences in blood serum detected by DSC and UV–VIS confirm a potential usefulness of these methods for diagnostic and monitoring changes with age as well as the pathological state of blood serum.

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smoking. The aim of the present study was to investigate the influence of maternal smoking during pregnancy on the early physical and neurobehavioral development of newborn rats. Our hypothesis was that maternal smoking during pregnancy interferes with

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Newborn rats of both sexes were treated (imprinted) with 20mg of benzpyrene. Two hours, 2 days, 1, 2, 3 weeks, 1 month and 2 months after imprinting the liver glucocorticoid receptors were studied for binding of dexamethasone. Two-hour and 2-day values were not appreciable. One week after treatment the receptor's affinity was extremely low both in control and treated treated animals. Two weeks after imprinting a significant difference in density (lower) and affinity (higher) was observed between the male treated and control animals. At 3 weeks and one month the binding capacity of treated and control animals was equal however, at 2 months Bmaxof males increased and that of females decreased significantly in the neonatally benzpyrene treated animals. This means that for the development of perinatal imprinting effect a long time is needed, and the effect is manifested after a period of lability.

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In earlier experiments single benzpyrene treatment of newborn rats caused strong alterations in the endorphin content of adult rats' immune cells. In the present experiments young (4-6 weeks old) male rats were studied for demonstrating the effect of the single neonatal or repeated (neonatally and at weanling) benzpyrene exposure on the serotonin content of immune cells (blood lymphocytes, monocytes, granulocytes; peritoneal fluid lymphocytes, mast cells, monocytes and granulocytes, thymic lymphocytes). Flow cytometric analysis showed that 50 µg benzpyrene treatment of five-week-old animals was ineffective after 5 days and this was the situation four weeks after single neonatal (20 µg) benzpyrene exposure. However, the repeated treatment of neonatally benzpyrene exposed 4 weeks old animals after 5 days resulted in elevated blood and thymic lymphocyte serotonin amount and in one index (peritoneal monocyte-granulocyte group) reduced serotonin content. This means that neonatal benzpyrene treatment does not influence directly the serotonin content (production or transport) of immune cells (unlike to the endorphin content) however, sensitizes them to a following benzpyrene exposure. The results widen the list of harmful effects (influencing steroid receptor binding, sexual behavior and immune cells' endorphin content) of perinatal benzpyrene exposure.

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. J., et al.: Glucagon-like-peptide-1 and exendin-4 stimulate β-cell neogenesis in streptozotocin-treated newborn rats resulting in presistenly improved glucose homeostasis at adult age

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58 41 50 Pállinger, É., Kovács, P., Csaba, G.: Presence of hormones (triiodothyronine, serotonin and histamine) in the immune cells of newborn rats

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. 24 407 414 Csaba G, Kovács P, Pállinger É: Single treatment (hormonal imprinting) of newborn rats with serotonin increases the serotonin content of

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23 1547 1558 Franchi G, Veronesi C: Long-term motor cortex reorganization after facial nerve serving in newborn rats. Eur. J. Neurosci. 20(7), 1885

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neurones from newborn rats. J. Neurophysiol. 79, 670-676 (1998) Inflammatory mediators at acidic pH activate capsaicin receptors in cultured sensory neurones from newborn rats J. Neurophysiol

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1991; 66: 153–156. 36 Nagy SU, Csaba G. Dose dependence of the thyrotropin (TSH) receptor damaging effect of gonadotropin in the newborn rats. Acta Physiol Hung. 1980; 56: 417

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