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Raw materials quality assessment is a very important factor, enabling products creation up to the international standards. In this investigation, the qualitative composition of bioactive plant extracts has been checked. The tested extracts have been obtained by the use of supercritical carbon dioxide extraction and can be utilized as raw materials in a commercial production of a wide range of many different goods. Plant extracts of raspberry (Rubus idaeus), strawberry (Fragaria ananassa), blackcurrant (Ribes nigrum), aronia (Aronia Medik.), Japanese rose (Rosa rugosa Thunb.) seeds, and palmetto palm (Sabal minor) fruit have been examined. In these extracts, saturated, monounsaturated, and polyunsaturated fatty acids as well as polyphenols have been identified using thin-layer chromatography (TLC) with aqueous-organic mobile phases. Appropriate content of these components tends to be a determinant of human organism operation. In our investigation, the plant extract components, especially saturated and unsaturated fatty acids and polyphenols, have been tested using micellar chromatography. Different micellar environments, containing polioxyethylene (23) lauryl ether (Brij35), sodium dodecyl sulfate (SDS), and cetyltrimethylammonium bromide (CTAB) as a main component of micellar mobile phases, have been utilized. The analyte-micelle association constants (K ma) from Foley’s equation have been compared for each of these environments.

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Abdallah, M. D., Kandil, M. A. and Farag, A. A. (1986): Bioactivity of plant extracts against Sitophilus granarius (L.) and Tribolium castaneum (Hbst.). Bull. Entomol. Soc. Egypt, Econ. Ser. 15, 199

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. and Alam , M. S. ( 2005 ): Antifungal effects of plant extracts on seedborne fungi of wheat seed regarding seed germination, seedling health and vigour index . Pakistan J. of Biological Sciences 8 , 1284 – 1289 . Heweidy , M. A. M. and

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., Piližota, V., Hribar, J. & Simčič M. (2009a): Total phenol content and antioxidant activity of water solutions of plant extracts. Croat. J. Fd Sci. Technol. , 1 , 1–7. Simčič M

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. , Esmaeili , M. A. , Dehghan , H. , Ghazi , I. ( 2013 ) α-Glucosidase and α-amylase inhibitory effect and antioxidant activity of ten plant extracts traditionally used in Iran for diabetes . J. Med. Plant. Res. 7 , 257 – 266 .

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Abstract  

Instrumental neutron activation analysis was applied to the determination of the elements Br, Ca, Cl, Cs, Fe, K, La, Mg, Mn, Na, Rb and Zn in medicinal extracts obtained fromCentella asiatica, Citrus aurantium L., Achyrolcline satureoides DC, Casearia sylvestris, Solano lycocarpum, Zingiber officinale Roscoe, Solidago microglossa andStryphnondedron barbatiman plants. The elements Hg and Se were determined using radiochemical separation by means of retention of Se in HMD inorganic exchanger and solvent extraction of Hg by bismuth diethyldithiocarbamate solution. Precision and accuracy of the results were evaluated by analyzing biological reference materials. The therapeutic action of some elements found in plant extracts analyzed is briefly discussed.

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Abdel-Monaim, M. F., Abo-Elyousr, K. A., Morsy, K. M. (2011) Effectiveness of plant extracts on suppression of damping-off and wilt diseases of lupine ( Lupinus termis Forsik). Crop Prot. 30 , 185

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The main volatile compounds from three medicinal plants belonging to Lamiaceae family were screened for their biological properties. The plants were Salvia officinalis, Thymus vulgaris, and Mentha × piperita containing as the main volatile constituents thujone, thymol, and menthol, respectively. The applied chromatographic system was silica gel developed with toluene-ethyl acetate (93:7). Thin-layer chromatography — direct bioautography (TLC-DB) against Escherichia coli and Bacillus subtilis was used for detection of antibacterial activity of the plant extracts and essential oils. The bioautographic fingerprints were compared with the fingerprints obtained after derivatization with anisaldehyde.

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As it was presented in our previous works, applying external magnetic field during chromatogram development results in changes of retention of chromatographed solutes which can be used for adjustment of their chromatographic behavior, in order to optimize the separation. In this paper, we would like to present the influence of application of external magnetic field during chromatogram development on thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) plates on separation of ingredients of plant extracts, which are multicomponent mixtures and are very difficult to separate under conventional chromatographic conditions.

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Summary

The quantification of the main withanolide, withaferin-A, in plant extracts of Withania somnifera and Withania coagulans using high-performance liquid chromatography (HPLC)-mass spectrometry (ESI-MS/MS) is described. HPLC was used in combination with a triple quadrupole mass spectrometer in multiple reaction monitoring (MRM) mode. Additionally, mass exact determination of withaferin-A was achieved using a hybrid high-resolution mass spectrometer (Q-TOF). A combination of acetonitrile and water as the mobile phase using gradient elution was found to be successful in separating the compound of interest from the other main components. The limit of detection (LOD) in the plants was 6 ng/g and the total analysis time was 10 min.

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