In this paper we describe a sensitive and reproducible reversed-phase high-performance liquid chromatography (HPLC) method with photodiode-array detection for isolation and quantification of the bioactive hydrophilic constituent 7-(1-O-β-d-galacturonide-4′-(1-O-β-d-glucopyranosyl)-3′,4′,5,7-tetrahydroxyflavone, 1, from the seeds of Cuminum cyminum. Compound 1 was separated isocratically on a C18 preparative column, in high purity, after removal of solvents. The purity and identity of the compound were established by use of LC-mass spectrometry and by spectroscopic techniques (1H and 13C NMR). The purity of 1 was also confirmed by HPTLC.
two classical methods that were applied. However, these two conventional strategies are time-consuming, labor-intensive, and expensive process. Recently, preparative high-performance liquid chromatography (prep-HPLC) has been used for separating
ultimately reducing library synthesis time to 3 days (Figure 2 ) [ 16 ]. After completion of the reactions, crude mixtures (directed by automation) can be purified by organic and/or aqueous prepHPLC. An active splitter can then send compound for analysis