A simple reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for simultaneous analysis of three triterpene acids (corosolic, oleanolic, and ursolic acids) in extracts from inflorescences, leaves, and fruits of Prunus serotina Ehrh. (American black cherry). Separation of the acids was accomplished on a C18 column (5 μm, 250 mm × 4.6 mm i.d.) and recorded at 210 nm. The greatest resolution was achieved with 90:10 (υ/υ) methanol-1% aqueous orthophosphoric acid as mobile phase at a flow rate of 0.6 mL min−1. The correlation coefficients for all the calibration plots (r > 0.998) showed linearity good over the range tested. The relative standard deviation of the method was less than 3.3% for intra and inter-day assays, and average recovery was between 95.9 and 100.9%. Sensitivity was high; detection limits were between 0.034 and 0.067 μg mL−1. Total amounts of triterpene acids were 0.451–0.928, 0.031, and 0.911–1.455% in the inflorescences, fruits, and leaves of P. serotina, depending on the time of harvesting.
Previous overviews of plant invasion in Hungary were based on local case studies and the authors’ experience. The MÉTA survey provided an opportunity to outline a more exact picture based on the survey of the whole country. This paper summarises the basic statistics related to plant invasion: cover of invaded area estimated for the country, each geographical region and each distinguished (semi-)natural habitat category, and cover of the selected 15 alien species in each habitat category.
The new Dutch forest monitoring network is a policy-guided, multiple-use, GIS-oriented forest monitoring network. It is designed to provide the Dutch government, on a cyclic 8-year basis, with actual information about Dutch forests. Variables that reflect the information needs of policy makers and interest groups were selected by means of interviews and workshops. High-ranking variables are: wood stock, ownership, stand age, management status, biodiversity, carbon stock, and recreational use. These and other variables are being measured in 3622 forest sites, selected according to an unaligned systematic sampling design. The data are stored in an ORACLE data base, made accessible through the internet. The Dutch forest area approximates a total of 360 000 ha; 46% is owned by private owners and organisations for nature conservation. Coniferous forests dominate (60%). Most forests were planted in 1940-1980. The total above ground volume of living trunk wood amounts to 56.3 million m3. The most common tree species are Quercus robur, Pinus sylvestris, and Betula pendula, the most common shrubs are Sorbus aucuparia, Prunus serotina, and Rhamnus frangula; while the most common other plant species are Deschampsia flexuosa, Rubus fruticosus s.l., and Dryopteris dilatata.
Downey, S. L., Iezzoni, A. F. (2000): Polymorphic DNA markers in black cherry ( Prunusserotina ) are identified using sequences from sweet cherry, peach, and sour cherry. J. Amer. Soc. Hort. Sci. , 125 , 76
Chabrerie, O., Verheyen, K., Saguez, R. and Decocq, G. 2008. Disentangling relationships between habitat conditions, disturbance history, plant diversity, and American black cherry (
Ehrh.) invasion in a European temperate forest