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carrying the wheat high molecular weight (HMW) glutenin Bx17 subunit and it use as an RFLP marker. Theor. Appl. Genet. 85 :616–624. Appels R. Analysis of a genomic DNA segment

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One-step RT-PCR followed by a RFLP assay was developed for the typing of Barley yellow dwarf virus (BYDV) member of Luteovirus genera. RFLP analysis performed on 23 samples including one lab isolate showed three types of Hpa II restriction profile. A partial coat protein (CP) gene sequencing was carried out and confirmed the RFLP analysis. Both sequencing and RFLP analysis identified the presence of 3 BYDV-PAV (including the lab isolate Blatno85), 10 BYDV-MAV and 10 BYDV-PAS isolates among the field samples. One-step RT-PCR together with RFLP presents an easy and reliable assay for routine BYDV typing. These methods revealed that PAS and MAV are more dominant than PAV in the Czech Republic.

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avian Pasteurella multocida isolates by PCR-RFLP of omp H gene. Iran. J. Biotechnol. 3 , 99–103. Esmaelizardeh M. Molecular typing of avian Pasteurella multocida isolates by PCR-RFLP

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Acta Alimentaria
Authors: J. Krulj, N. Ćurčıć, A. Bočarov Stančıć, J. Kojıć, L. Pezo, L. Peıć Tukuljac, and M. Bodroža Solarov

sequences for the description and identification of the species using a multilocus approach. PCR-restriction fragment length polymorphism (PCR-RFLP) is a simple, cost effective and quick tool for rapid detection of specific differences in DNA sequences of

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. Lewis Publishers Inc., pp. 35-37 and 93-137. 35 37 Krause, D., Szibor, R., Kuchhenser, W., Brückner, R.: The practical significance of human genetic RFLP

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combined PCR-RFLP method. Dis. Aquat. Org. 44, 35--39. Identification of fish parasitic Myxo-bolus (Myxosporea) species using a combined PCR-RFLP method. Dis. Aquat. Org

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Footrot is widely considered the most severe and most common foot pathology in small ruminants. This study tested the ability of a molecular typing system based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay of the 16S rRNA gene to discriminate between the strict anaerobe genera most commonly isolated from footrot ( Bacteroides, Dichelobacter, Fusobacterium, Porphyromonas and Prevotella ) in goats in Extremadura (Spain), with a view to facilitating identification for diagnostic purposes and thus providing a useful tool for future epidemiological studies. Although the electrophoretic patterns obtained with the enzyme Tru 1I were more readily interpreted, and may thus be the best initial option, results may be confirmed by a second enzyme ( Rsa I). The PCR-RFLP assay of the 16S rRNA gene may therefore prove a useful addition to conventional biochemical identification techniques, providing taxonomic information at genus level.

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Acta Veterinaria Hungarica
Authors: Zsuzsanna Tapaszti, Petra Forgách, Csaba Kővágó, László Békési, Tamás Bakonyi, and Miklós Rusvai

Microsporidiosis (nosema disease) of the European honeybee ( Apis mellifera L.) is present in bee colonies worldwide. Until recently, Nosema apis had been regarded as the causative agent of the disease, which may have many negative effects on the colony and cause heavy economic losses in apicultures. Another microsporidium species, Nosema ceranae , was reported to infest the Asian honeybee ( Apis ceranae ), but both honeybee species are susceptible to both microsporidia. In the European honeybee N. ceranae was first detected in Spain in the year 2006. As it is difficult to distinguish N. ceranae and N. apis morphologically, a rapid and accurate assay has been developed to differentiate N. apis and N. ceranae based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the partial large subunit ribosomal RNA. The assay was tested on 38 Nosema -infested bee samples, which were collected from geographically distant Hungarian bee colonies representing all regions of the country. Only one sample contained N. apis , and in the other 37 samples N. ceranae was detected, which indicates the dominance of N. ceranae in Hungarian apiaries. This is the first report on the presence of N. ceranae in Hungary.

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Jahoor, A., Jacobi, A., Schüller, C. M. E., Fischbeck, G. 1993: Genetical and RFLP studies at the Mla locus conferring powdery mildew resistance in barley. Theor. Appl. Genet. , 85 , 713-718. Genetical and RFLP studies at the

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Wine fermentation is a complex microbiological process in which yeasts predominate. It is long debated whether yeasts occurring on the surface of grapes or the resident yeasts on the winery equipment play the primary role in conducting the fermentation. The origin, development, changes and succession of various yeast species can be followed using specific molecular techniques allowing the differentiation and typing of yeast strains. Techniques such as pulsed field gel electrophoresis of chromosomal DNA, restriction fragment length polymorphism analysis, and polymerase chain reaction (PCR)-based methods have recently been employed in studying the microbiology of wine making. These shed new light on the dynamics of fermentation started spontaneously or directed by the inoculation of starter cultures.

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