Authors:S. Naeimi, S. Kocsubé, Zsuzsanna Antal, S. Okhovvat, M. Javan-Nikkhah, C. Vágvölgyi, and L. Kredics
Hermosa, M. R., Grondona, I., Diaz-Minguez, J. M., Iturriaga, E. A., Monte, E. (2001) Development of a strain-specific SCARmarker for the detection of Trichoderma atroviride 11, a biological control agent against soilborne fungal plant pathogens. Curr
Authors:J. Jia, G. Li, C. Liu, M. Lei, and Z. Yang
Wheat yellow rust resistance gene Yr17 was originated from the wheat-Aegilops ventricosa introgression, and still effective on the adult plant in Southern China. The previous studies located the gene Yr17 on the translocation of 2NS-2AS using the molecular and cytological markers. In the present study, we screened new PCR-based markers to map the gene Yr17 region from the investigation of a segregating 120 F2 population. All markers including four EST-PCR markers, a SCAR (sequence characterized amplified region) and a PLUG (PCR based landmark unique gene) marker specific to Yr17 gene were mapped on the chromosome 2AS, and located on the chromosomal deletion bin 2AS5-0.8–1.00 region. Based on the wheat-rice collinearity, we found that the sequences of the Yr17 gene linked markers were comparatively matched at rice chromosome 4 and chromosome 7. However, the identified closely linked genomic sequence of Yr17 gene is most likely collinear with genomic region of rice chromosome 4. The newly produced PCR based markers closely linked to Yr17 gene will be useful for the marker-assisted selection in wheat breeding for rust resistance.
Authors:L. Błaszczyk, I. Kramer, F. Ordon, J. Chełkowski, M. Tyrka, G. Vida, and I. Karsai
Gupta, S.K., Charpe, A., Koul, S., Haq, Q.M.R., Prabhu, K.V. 2005a. Development and validation of SCARmarkers co-segregating with an
derived leaf rust resistance gene
in wheat. Euphytica
Authors:J. Saju, Sz. Németh, Réka Szűcs, Rashmi Sukumaran, Z. Lim, L. Wong, L. Orbán, and M. Bercsényi
The identification of three scorpionfish species, the black scorpionfish (Scorpaena porcus Linnaeus, 1758), the large-scaled scorpionfish (S. scrofa Linnaeus, 1758) and the small red scorpionfish (S. notata Rafinesque, 1810) is possible in adults by morphometry, but often problematic in juveniles due to their similar phenotypes. To develop a molecular species identification tool, first, we have analyzed the genetic similarity of the three species by a PCR-based ‘blind method’ that amplified bands from various locations of the genome. We found high levels of nucleotide similarity between S. porcus and S. scrofa, whereas S. notata showed a higher level of divergence from the other two species. Then, we have searched these patterns for differences between the genomes of Adriatic specimen of these three species and identified several species-specific products in two of them. For the third one a species-specific primer pair amplifying from the 16S ribosomal DNA was designed. One marker for each species was cloned, sequenced and converted into Sequence Characterized Amplified Region (SCAR) markers amplified by specific primer pairs. The SCAR markers amplified robust bands of limited variability from the target species, while no or only occasional weak products were obtained from the other two, proving that they can be used for molecular identification of these three species. These markers can help the conservation and future analysis of these three species as well as their possible selection programs for aquaculture purposes.
Authors:C. Liu, Z. Yang, J. Jia, G. Li, J. Zhou, and Z. Ren
Knowledge of the chromosomal distribution of long terminal repeats (LTR) is important for understanding plant chromosome structure, genomic organization and evolution, as well as providing chromosomal landmarks that are useful for chromosome engineering. The aim of this study is to investigate the genomic distribution of
-like LTR pDbH12, which was first isolated from
species in relation to the genomic evolution and chromosome identification. Fluorescence
hybridization (FISH) analysis showed that pDbH12 is present on
(V genome) and
(H genome) species with the hybridized signals covering the entire chromosomes. However, clone pDbH12 did not hybridize to the genomes of
Secale, Triticum, Lophopyrum, Pseduoroengeria, Aegilops, Agropyron desertorum
Elymus. Thinopyrum intermedium
displayed fourteen chromosomes that hybridized with pDbH12. Sequential FISH identified these chromosomes as belonging to the J
genome. Results from sequence characterized amplified region (SCAR) marker and dot blot both support the FISH results, and the integrative results suggest that amplification of
-like LTR retrotransposons is an important factor which involved in the speciation process. Clone pDbH12 could serve as a cytogenetic marker for tracing chromatin from V or V
, H and J
genomes in wheat-alien introgression lines.