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Cereal Research Communications
Authors: X. Zhang, Y. Chen, Y. Wei, W. Lu, H. Liao, Y. Liu, X. Yang, X. Li, L. Yang, L. Li, and R. Li

mapping of 2240 new SSR markers for rice ( Oryza sativa L.). DNA Research 9 :199–207. Stein L. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L

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Grain Protein Content (GPC) of wheat is significant for enhanced nutritional value and is one of the most important aspects effecting pasta and bread making quality as well. Seventy seven Pakistani wheat varieties and advance lines were analysed to access the allelic distribution at microsatellite Xuhw89 locus using functional SSR marker. Overall, 42% of tested wheat genotypes were found to carry 126 + 130-bp allele while a 126-bp allele was detected in 58% of genotypes. A target band of 126-bp was amplified in all tested genotypes, however, an additional band of 130-bp was also detected along with 126-bp band in 32 genotypes. Genotypes i.e. (Punjab-96) total soluble protein and (MEXI PAK) globulin with 126 + 130-bp allele while (Faisalabad-2008) salt soluble protein and (TC-4928) albumin with allele of 126-bp depicted highest grain protein content. The alleles identification associated with maximum grain protein content in Pakistani wheat germplasm will assist in accelerating the breeding program in future.

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., Weibull, J. 2007. Genetic diversity changes and relationships in spring barley ( Hordeum vulgare L.) germplasm of Nordic and Baltic areas as shown by SSR markers. Genet. Resour. Crop Evol. 54 :749

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. 2013 . Genetic diversity in Algerian maize (Zea mays L.) landraces using SSR markers . Maydic . 58 : 304 – 310 . Akinwale , R. , Badu-Apraku , B. , Fakorede , M. , Vroh-Bi , I

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Danin-Poleg, Y., Tzuri, G., Reis, N., Katzir, N. (1998): Application of inter-SSR markers in melon ( Cucumis melo L.). Cucurbit. Genet. Coop. Rep. , 21 , 25–28. Katzir N

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699 707 Iqbal, N., Firdissa, E., Khlestkina, K., Weidner, A., Röder, M.S., Börner, A. 2007. The use of simple sequence repeat (SSR) markers to identify and map alien segments

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, and SSR markers and its effect on the b-glucan content of wheat. Genome 54 :1–10. Molnár-Láng M. Characterization of a new 4BS.7HL wheat-barley translocation line using GISH, FISH

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Plant Nutr. Ferti. Sci. 2004 10 473 478 Ciucă, M., Petcu, E. (2009) SSR markers associated

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1114 Song, Q. J., Shi, J. R., Singh, S., Fickus, E. W., Costa, J. M., Lewis, J., Gill, B. S., Ward, R., Cregan, T. B. (2005): Development and mapping of microsatellite (SSR) markers in wheat

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Genetic structure of 142 parent lines of sorghum [Sorghum bicolor (L.) Moench] was analyzed using model-based approach based on SSR markers. Forty-one selected from 103 SSR markers were used to analyze the parent lines, which generated 189 alleles revealed by each marker ranging from 2 to 11 with an average of 4.6 per marker. The polymorphic information content (PIC) value was 0.543 with a range of 0.089 to 0.850. All the parent lines were assigned to 7 subgroups, named Kafir, Kaoliang, Feterita, Shallu, Hegari, Milo and Durra. Parent lines without clear pedigree record were clustered into their corresponding groups, and genetic components of each line were estimated by Q-values. Information of this study would be useful for breeders to conclude their genetic background and select appropriate parents for germplasm improvement and hybrid breeding, and thus improve the efficiency of breeding programs.

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