A previous study on Solidago virgaurea extracts showed an inhibiting activity of Candida albicans yeast—hyphal transition due to a mixture of triterpene saponins, leading to applications in the field of oral care products. Such applications require the development of an efficient, fast, and simple quantification method of S. virgaurea total saponins. Two methods were developed: the first was based on high-performance liquid chromatography (HPLC) separation with gradient elution and evaporative light scattering detection (ELSD); the second was based on high-performance thin-layer chromatography (HPTLC) separation with in-situ hydrolysis followed by densitometric measurements at 366 nm. Both calibration curves showed good linear regressions (R2 > 0.99) within the range of the concentrations tested. The limit of detection (LOD) and the limit of quantitation (LOQ) were 2–4 μg and 10–16 μg, respectively. The intra- and inter-day variations were studied and found to remain below 5.6% in terms of relative standard deviation (RSD). The recoveries were 97.02–101.91% with RSD of 0.3–1.96% for spiked sample. Both validated methods were successfully applied to the analysis of total saponins in different S. virgaurea samples. In particular, a harvesting study could be supported by these methods to identify the most relevant vegetal parts to extract, and the locations and vintage to collect, to obtain the desired active saponins. HPLC is the recommended method for precise analyses, but HPTLC is the most efficient for the fast analysis of multiple samples.
Baranová , B. , P. Manko and T. Jászay . 2014 . Differences in surface-dwelling beetles of grasslands invaded and non-invaded by goldenrods ( Solidago canadensis, S. gigantea ) with special reference to Carabidae . J. Insect Conserv. 18 : 623 – 635
absence of other Hungarian transformers (such as Fallopia × bohemica, Robinia pseudoacacia and Solidago canadensis ) in the cited list ( FPS 2022 ), draws our attention to the special invasion biological situation of the Pannonian Basin and/or Central
Instrumental neutron activation analysis was applied to the determination of the elements Br, Ca, Cl, Cs, Fe, K, La, Mg, Mn, Na, Rb and Zn in medicinal extracts obtained fromCentella asiatica, Citrus aurantium L., Achyrolcline satureoides DC, Casearia sylvestris, Solano lycocarpum, Zingiber officinale Roscoe, Solidago microglossa andStryphnondedron barbatiman plants. The elements Hg and Se were determined using radiochemical separation by means of retention of Se in HMD inorganic exchanger and solvent extraction of Hg by bismuth diethyldithiocarbamate solution. Precision and accuracy of the results were evaluated by analyzing biological reference materials. The therapeutic action of some elements found in plant extracts analyzed is briefly discussed.
Solidago canadensis L., (Asteraceae) has been used in European phytotherapy as a urological and antiphlogistical remedy for centuries. The behaviour of dissolution of mineral elements into different tinctures and aqueous extracts obtained from Solidaginis herba was investigated in connection with their quercetin glycoside and organic acid amount. Commonly applied aqueous and alcoholic extracts were analysed for Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Li, Mg, Mn, Mo, Na, P, Pb, S, Ti, V and Zn content. The concentrations of the minerals examined were determined by inductively coupled plasma emission spectrometry (ICP-OES). Determination of the flavonoids in solidaginis herba and extracts was carried out by a spectrophotometric method, as required by the German Pharmacopoea. For the study of the flavonoid composition of crude drug, the HPLC technique was applied. To determine the relative nutrient contribution of these pharmaceuticals to the diet, data obtained were combined with flavonoid content particulars, then a comparison with U.S Recommended Dietary Allowances (RDA) was made. For evaluation of the phytotherapeutic relevance, k/Na ratio was also calculated. It has been found that the pharmaceuticals examined are important sources of potassium, chromium, manganese, calcium, magnesium, phosphorus and lower sources of iron and zinc, assuming a daily intake of 1-2 l aqueous extracts as recommended for urological diseases. Flavonoid content of the different Solidaginis herba extracts ranged from 62.4 mg l-1 to 305.2 mg l-1.