Singh, N. K., Donovan, G. R., Batey, I. L. and Mac Ritchie, F. (1990a): Use of sonication and size-exclusion high-performance liquid chromatography in the study of wheat flour proteins I. Dissolution of totalproteins in the absence of reducing agents
In this study, shocks of cold at the levels of 5,3 and 1oC have been applied to the 6-days old chickpea (Cicer arietinumL.) plants. Next, having been left to grow for 72 hours under long day photoperiod circumstances 25oC, the length of their root and epicotyl have been measured at certain intervals. Moreover, the proteincontents of the plants exposed to low temperature shocks (1oC) have been indentified in connection with their controls. According to the results obtained, the low temperature shocks have prevented the length of the roots and epicotyl to a large extent. It is observed that the lower the temperature is, the more the plant's growth is prevented. It has also been observed that the cold sterss caused by the low temperature application might be reversible. The plants get rid of this stress later in low temperatures than in high ones. The low temperature application (1oC) has also considerably reduced the total protein amount.
Flour from grains originating from plants infected artificially with cereal aphids were analyzed for glutenin and gliadin and total protein content, using Size Exclusion HPLC. Wheat plants were caged at the beginning of stem elongation. Cages were treated with 0.1 % methyl parathion. One week later, the caged plants were artificially infected with 5 aptera individuals of
Metopolophium dirhodum, Diuraphis noxia, Sitobion avenae
. It was found that aphid infection had significant effect on the glutenin and gliadin content, the total protein content and the gliadin/glutenin ratio. Both the glutenin and gliadin content was significantly higher in the seeds harvested from aphid infected plants. However, the gliadin/glutenin ratio was significantly lower in wheat flour prepared from aphid infected plants than in those from uninfected control. The most significant decrease in gliadin/glutenin ratio was caused by
M. dirhodum, D. noxia, S. avenae
infection followed by
at high-abundance. As the gliadin/glutenin ratio was significantly lower in flours made from aphid infected wheat seeds, it may be suggested, that aphid feeding results in decreased bread making quality of wheat flour.
Authors:A. S. Gerrano, M. T. Labuschagne, A. van Biljon, and N. G. Shargie
Sorghum is a drought tolerant cereal and staple food which is a dietary source of protein and more than 20 minerals. The concentration of the mineral elements and protein content in sorghum varies due to genotypic and environmental influences and genotype by environment interactions. The objective of this study was to determine the contents of eight mineral elements (Ca, Fe, K, Mn, Na, P, Zn and Mg) and protein in sorghum genotypes. The analysis of variance showed significant differences in mineral and protein contents. There was a significant relationship between Zn and Fe and between protein and P and Zn. The principal component (PC) analysis showed that Fe, Mn, P, Zn and protein contributed largely to clustering of the genotypes in PC1; Ca, P and Mg to PC2 and Ca, K and Na to PC3. The presence of a considerable amount of compositional variability of mineral and protein contents among tested genotypes suggests that they can be a valuable source of genes for nutritional quality improvement of sorghum.
Authors:Zita Faixová, Štefan Faix, Radka Bořutová, and Ľubomír Leng
Doumas, B. T., Bayse, D. D., Carter, R. J., Peters, T. Jr. and Schaffer, R. (1981): A candidate reference method for determination of totalprotein in serum. I. Development and validation. Clin. Chem.
mitoz bölünme ve totalprotein miktari üzerine etkisi. Turk. J. Biol. 24, 127-140.
Zeytinyagi fabrikasi atik suyunun bugday (Triticum aestivum L.) kök ucu hücrelrindeki mitoz bölünme ve totalprotein miktari üzerine etkisi
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totalprotein bound and nonprotein sulfhydryl group in tissue with Ellman's reagents Anal. Biochem. 25 192 – 205 .
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