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Acta Chromatographica
Authors: Weijian Ye, Wei Sun, Ruijie Chen, Zhe Wang, Xiao Cui, Hui Zhang, Shuyi Qian, Qi Zheng, Yangfeng Zhou, Jiafeng Wan, Jiali Xu, Xianqin Wang, and Yunfang Zhou

speed of chromatographic separations, with good resolution and sensitivity. A triple-quadrupole mass spectrometer is widely used in quantification due to its high sensitivity and selectivity. Therefore, UHPLC–MS/MS is becoming a useful technique for

Open access
Acta Chromatographica
Authors: Su-su Bao, Jian Wen, Teng-hui Liu, Bo-wen Zhang, Chen-chen Wang, and Guo-xin Hu

experiments about olmutinib will be done. However, a method for determination and pharmacokinetic investigation of olmutinib has not yet been reported. In this article, we describe a quantitative analysis of olmutinib using UHPLC–MS/MS

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could be used for the comparison of the pharmacokinetic characteristics between CCSG and calycosin. In this study, we used ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) combining multiple reaction monitoring (MRM

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-tandem mass spectrometry (UHPLC-MS/MS) is an excellent method for the selective and sensitive determination of small molecular weight compounds in biological samples [ 6, 7 ]. Fenofibrate is a prodrug that is hydrolyzed immediately after absorption by tissue

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Acta Chromatographica
Authors: Biljana K. Tubić, Bojan D. Marković, Sandra S. Vladimirov, Slavica M. Ristić, Branka M. Ivković, Miroslav M. Savić, Jelena M. Poljarević, and Tibor J. Sabo

A series of new (S,S)-ethylenediamine-N,N′-di-2-(3-cyclohexyl)propanoate esters has shown cytotoxic activity towards human leukemic cell lines. The aim of this study was to develop and validate a bioanalytical method for quantification of (S,S)-O,O-diethyl-ethylenediamine-N,N′-di-2-(3-cyclohexyl)propanoate dihydrochlorides (DE-EDCP) and its metabolite, substituted propanoic acid (EDCP), in mouse serum by ultra high-performance liquid chromatography—tandem mass spectrometry (UHPLC—MS/MS). Structural analog, derivative of 1,3-propanediamine, was used as an internal standard (IS). Sample preparation employed protein precipitation by acetonitrile and subsequent centrifugation. Optimal UHPLC separation conditions were set to achieve simultaneous determination of both compounds in a short run time of 6 min. Additionally, the selected reaction monitoring (SRM) mode developed in this method allowed a highly sensitive, accurate, and precise identification of compounds of interest. The lower limit of quantitation (LOQ) was 1.3 ng mL−1 for DE-EDCP and 0.3 μg mL−1 for EDCP. The calibration curves were linear over the concentration range of 1.3–26.7 ng mL−1 and 0.3–6.7 μg mL−1 for DE-EDCP and EDCP, respectively. Precision (%CV) and accuracy (%RE) for DE-EDCP and EDCP ranged from 3.5% to 16.0% and from 1.8% to 14.4%, respectively.

The validation process was performed in accordance with the regulatory guidance/guideline, and all of the obtained results met the established acceptance criteria. The newly developed and validated UHPLC—MS/MS method is rapid, sensitive, and selective, and it can be successfully applied to drug monitoring in nonclinical studies.

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sample extraction and dispersive SPE cleanup method was developed with UHPLC–MS/MS analysis for the simultaneous determination of 16 antibiotics in preserved eggs. The method was found to be rapid, safe, sensitive, and cheap and was applied to determine

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., 2015 ) and mycotoxins ( Dzuman et al., 2014; Qian et al., 2018 ) in feeds. UHPLC-MS/MS device has been extensively used for detection, identification, and quantification of multiclass antibiotic residues ( Boscher et al., 2010; Zhang et al., 2013; Qian

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detect acrylamide in water [ 25 ], but not used in the detection of CAP. In this study, an evaporation preparative step method with isotope dilution UHPLC–MS/MS has been developed for the determination of CAP in drinking water from Shannan region

Open access

-EGDMA) monolithic column combined with UHPLC-MS/MS for the determination of ligustrazine has been developed in rat serum. Relevant experimental parameters, such as pH of loading solution, loading volume, and desorption condition, were evaluated. Finally, the

Open access

References B eltrán , E. , I báñez , M. , S ancho , J.V. & H ernández , F. ( 2014 ): Determination of patulin in apple and derived products by UHPLC-MS/MS. Study of

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