the Quantitative Structure Retention Relationship (QSRR) approach [ 38 ]. Finally, based on the developed UHPLC-MS/MS method and the NMR studies, we managed to successfully separate and determine ziprasidone and its five main impurities, and to
speed of chromatographic separations, with good resolution and sensitivity. A triple-quadrupole mass spectrometer is widely used in quantification due to its high sensitivity and selectivity. Therefore, UHPLC–MS/MS is becoming a useful technique for
A series of new (S,S)-ethylenediamine-N,N′-di-2-(3-cyclohexyl)propanoate esters has shown cytotoxic activity towards human leukemic cell lines. The aim of this study was to develop and validate a bioanalytical method for quantification of (S,S)-O,O-diethyl-ethylenediamine-N,N′-di-2-(3-cyclohexyl)propanoate dihydrochlorides (DE-EDCP) and its metabolite, substituted propanoic acid (EDCP), in mouse serum by ultra high-performance liquid chromatography—tandem mass spectrometry (UHPLC—MS/MS). Structural analog, derivative of 1,3-propanediamine, was used as an internal standard (IS). Sample preparation employed protein precipitation by acetonitrile and subsequent centrifugation. Optimal UHPLC separation conditions were set to achieve simultaneous determination of both compounds in a short run time of 6 min. Additionally, the selected reaction monitoring (SRM) mode developed in this method allowed a highly sensitive, accurate, and precise identification of compounds of interest. The lower limit of quantitation (LOQ) was 1.3 ng mL−1 for DE-EDCP and 0.3 μg mL−1 for EDCP. The calibration curves were linear over the concentration range of 1.3–26.7 ng mL−1 and 0.3–6.7 μg mL−1 for DE-EDCP and EDCP, respectively. Precision (%CV) and accuracy (%RE) for DE-EDCP and EDCP ranged from 3.5% to 16.0% and from 1.8% to 14.4%, respectively.
The validation process was performed in accordance with the regulatory guidance/guideline, and all of the obtained results met the established acceptance criteria. The newly developed and validated UHPLC—MS/MS method is rapid, sensitive, and selective, and it can be successfully applied to drug monitoring in nonclinical studies.
experiments about olmutinib will be done. However, a method for determination and pharmacokinetic investigation of olmutinib has not yet been reported. In this article, we describe a quantitative analysis of olmutinib using UHPLC–MS/MS
could be used for the comparison of the pharmacokinetic characteristics between CCSG and calycosin. In this study, we used ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) combining multiple reaction monitoring (MRM
-tandem mass spectrometry (UHPLC-MS/MS) is an excellent method for the selective and sensitive determination of small molecular weight compounds in biological samples [ 6, 7 ]. Fenofibrate is a prodrug that is hydrolyzed immediately after absorption by tissue
., 2015 ) and mycotoxins ( Dzuman et al., 2014; Qian et al., 2018 ) in feeds. UHPLC-MS/MS device has been extensively used for detection, identification, and quantification of multiclass antibiotic residues ( Boscher et al., 2010; Zhang et al., 2013; Qian
sample extraction and dispersive SPE cleanup method was developed with UHPLC–MS/MS analysis for the simultaneous determination of 16 antibiotics in preserved eggs. The method was found to be rapid, safe, sensitive, and cheap and was applied to determine
was simple synthesised via self-assembly strategy with a direct carbonisation process. Subsequently, an efficient and novel analytical methodology based on MOMC and MSPE, coupled with UHPLC-MS/MS, was applied to the trace analysis of four acid