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Acta Chromatographica
Authors:
Shaoshi Wen
,
Zixin Zhang
,
Xiaopeng Chen
,
Jinchang Liu
,
Haiyang Yu
,
Lifeng Han
,
Lijun Jin
,
Yi Zhang
, and
Tao Wang

to keep retention time and resolution, which is unsuitable for most chromatographic columns. In the past decade, ultra-performance liquid chromatography (UPLC) is of high sensitivity, short analysis time, and good resolution, which has been

Open access

approach like ultra-performance liquid chromatography (UPLC) can enhance greener performance by decreasing solvent consumption and waste generation per single analysis. Using miniaturized techniques like UPLC covers 11 out of 12 green analytical principles

Open access
Acta Chromatographica
Authors:
Yuping Shen
,
Minhui Xu
,
Peipei Deng
,
Qinying Gu
,
Huawu Yin
,
Guohua Xia
,
Xiaobin Jia
,
Huan Yang
, and
James Tam

components in food materials, that is, ultra-performance liquid chromatography (UPLC) method was established to quantify the flavonol glycosides. Furthermore, linearity, precision, accuracy, stability, and repeatability of the established UPLC method were

Open access

Summary

A simple and rapid ultra-performance liquid chromatographic (UPLC) method for analysis of parabens in cosmetic products has been developed and validated. Four preservatives (methylparaben, ethylparaben, propylparaben, and butylparaben) were extracted by ultrasonication with methanol. Separation was achieved by use of a C18 column (2.1 mm × 50 mm i.d., 1.7 μm particle size) and gradient elution with methanol-water-phosphoric acid as mobile phase. Photodiode-array (PDA) detection was used and quantification with MaxPlot was in the range 210–400 nm. Regression analysis revealed linear relationships between peak area and amount of each additive from 0.1 to 10 μ mL−1, with correlation coefficients between 0.9971 and 0.9983. Inter-day and intraday precision for the four preservatives at three concentrations were 0.04–0.05% for retention time and 0.81–1.29% for peak area. Recovery was from 91.4–105.8%. The method was used for analysis of parabens in cosmetics and was proved suitable for rapid and reliable quality control.

Full access
Acta Chromatographica
Authors:
E.N. Fedorova
,
A.D. Kivero
,
N.V. Geraskina
, and
L.R. Ptitsyn

A simple and rapid ultra-performance liquid chromatographic (UPLC) method for analyzing acetoin in bacterial culture fluid was developed and validated for the first time. The samples were separated using an Acquity BEH C18 column (2.1 mm × 100 mm, 1.7 μm particle size) and isocratic elution with 30 mM phosphoric acid—1% acetonitrile as the mobile phase. A photodiode array detector (PDA) was used. The run time was 6 min, and the detection limit was 2.11 × 10−4 mg mL−1. The UPLC method was compared with high-pressure liquid chromatography (HPLC) for acetoin analysis. The proposed UPLC method is highly sensitive and was successfully applied to the analysis of acetoin in bacterial culture fluid.

Open access

Summary

An ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry UPLC-QTOF-MS method has been developed for qualitative and quantitative analysis major compounds in Saussurea eopygmaea Hand-Mazz, which has long been used as a traditional Tibetan medicine. This method was validated to be sensitive, precise, and accurate with the limits of detection of compounds 2,3,4,6, and 7 with 0.67–1.90 μg mL−1, the overall intra-day and inter-day variations less than 8.45%, and the overall recovery over 93.8%, respectively. The correlation coefficients (R 2) of the calibration curves were higher than 0.998. In addition, by comparison MS and MS/MS spectra with those of authentic compounds and literatures, a total of 14 main peaks were identified within 6 min. These results demonstrate that this approach has the potential for quality control of S. eopygmaea and other Tibetan herbal medicines.

Open access

Summary

A new ultra-performance liquid chromatographic (UPLC) method has been developed and validated for quantification of substances related to varenicline tartrate, process-related and degradation products, in pharmaceutical formulations. Chromatographic separation of six impurities was performed on a reversed phase column. The method was validated for linearity, limits of detection and quantification, accuracy, precision, and selectivity. The calibration plots obtained for the six impurities were linear over the range 0.005–0.30%. The relative standard deviations (s r) of intra and inter-day experiments were less than 1.0%. The detection limits ranged between 0.002 and 0.004%, depending on the impurity. The proposed UPLC method was successfully applied to quantification of varenicline impurities in its pharmaceutical formulation.

Open access
Acta Chromatographica
Authors:
Mengya Lu
,
Qianqian Tang
,
Chenyu Zhou
,
Zhizheng Fang
,
Zheng Fan
,
Xiangyu Li
,
Rongchun Han
, and
Xiaohui Tong

) [ 18–20 ], mass spectrometry (MS) [ 21 , 22 ], gas chromatography (GC) [ 23 ,  24 ], high-performance liquid chromatography (HPLC) [ 25–27 ], nuclear magnetic resonance (NMR) [ 28–31 ] and high-performance liquid chromatography (UPLC) [ 32–34 ] are

Open access

A new ultra-performance liquid chromatography method for the simultaneous determination of valsartan, amlodipine besylate, and hydrochlorothiazide in pharmaceutical formulations has been developed. Chromatographic separation was achieved within 1 min on a sub-2 μm RP18 column using an isocratic mode mobile phase consisting of a mixture of methanol and phosphate buffer (0.05 M, pH 3 ± 0.05) in the ratio 70:30 (v/v). The flow rate was set at 0.3 mL min−1 with a detection wavelength of 239 nm. The method was validated over the concentration ranges from 6.5 to 15.0 μg mL−1 for hydrochlorothiazide, from 5.0 to 12 μg mL−1 for amlodipine besylate and from 76.5 to 178.5 μg mL−1 for valsartan. Calibration plots were linear for valsartan, amlodipine besylate, and hydrochlorothiazide with a correlation coefficient greater than 0.99. Relative variation coefficients for repeatability and reproducibility were less than 3.0%. Recoveries from standard addition essay of individual component in pharmaceutical formulations were varied from 97.0% to 102.0%. The proposed method was successfully applied for the determination of valsartan, amlodipine besylate, and hydrochlorothiazide in pharmaceutical formulations with recoveries with respect to label amount in pharmaceutical formulations that varied from 98.0% to 102.9%. The low flow rate, short analysis time, and simple mobile phase composition make the method cost effective, rapid, nontedious, and successfully employed for simultaneous determination of these drugs in commercial pharmaceutical products.

Open access
Acta Chromatographica
Authors:
Yongxi Jin
,
Yuyan Chen
,
Jiawen Liu
,
Xi Bao
,
Yinghao Zhi
,
Congcong Wen
, and
Wenzong Zhu

–tandem mass spectrometry (UPLC–MS/MS) reported for the determination of ebeiedinone. To the best of our knowledge, the pharmacokinetics of ebeiedinone had not been reported. In this paper, UPLC–MS/MS method was established to determine ebeiedinone in mouse

Open access