Authors:Cs. Dobolyi, F. Sebők, J. Varga, S. Kocsubé, G. Szigeti, N. Baranyi, Á. Szécsi, B. Tóth, M. Varga, B. Kriszt, S. Szoboszlay, C. Krifaton and J. Kukolya
Borisova, L., Tasheva, Y. & Sertova, N. (2008): Investigation on the contamination of wheat and maize with Aspergillus flavus and determination of its toxicological properties to produce aflatoxins. Vet. Med-US , 12 , 60
. and Beltz , S. B. ( 2005 ): Characterization of aflatoxin-producing fungi outside of Aspergillus section Flavi . Mycologia 97 , 425 – 432 .
Criseo , G. , Bagnara , A. and Bisignano , G. ( 2001 ): Differentiation of aflatoxin
Authors:P. Rauch, L. Fukal, J. Prošek, P. Březina and J. Káš
Radioimmunoassay for the differential determination of aflatoxin M1 has been developed. It is based on the use of antiserum having almost the same affinity to both aflatoxins B1 and M1 /Ka for aflatoxin B1=2.0×109 and Ka for aflatoxin M1=2.1×109/ and making possible their simultaneous determination. Aflatoxin B1 content is determined specifically by a commercial RIA, and aflatoxin M1 concentration is calculated as the differences between these two assays.
Authors:Anita Risa, Dalma Maja Divinyi, Erzsébet Baka and Csilla Krifaton
Aflatoxin B1 (AFB1) is one of the most harmful mycotoxins occurring almost worldwide. Aflatoxins are a group of several compounds mainly produced by Aspergillus flavus and Aspergillus parasiticus [ 1 ] but more
The specificity of radioimmunoassay of aflatoxin B1 was tested. Relative cross-reactivity of used antiserum with aflatoxins B1, B2, G1, G2 and M1 was found to be 100%, 24%, 44.2%, 10.3%, and 1.4%, respectively. The interference of coumarin, albumins, steroids and ethylvanilin was estimated also in radioimmunoassay of aflatoxin B1. Thus these compounds may cause a false positive finding of aflatoxin B1.