Search Results

You are looking at 1 - 10 of 87 items for :

  • "allelic variation" x
  • All content x
Clear All

50 669 679 Gupta R.B., Singh N.K., Shepherd K.W. 1989. The cumulative effect of allelic variation in LMW and HMW glutenin subunits on dough

Restricted access

Gupta, R.B., MacRitchie, F. 1994. Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1 of common wheats. II. Biochemical basis of the allelic effects on dough properties. J. Cereal Sci. 19 :19

Restricted access

Sixty durum wheat genotypes were analysed for protein (gliadin) polymorphism to find out the existing genetic diversity, and to assess its utility for improvement in grain yield along with quality traits. Six different Gli-B 1 alleles were found in land races, rust resistance sources and old released varieties, while two in recently released and advance lines. Most of the recently released varieties and advance lines showed γ-45/ Gli-1 alleles, which is the best type for pasta making quality, remaining showed γ-42/ Gli-1 allele, which are not good for pasta making. It is advisable to select for γ-45/ Gli-1 as a bio-chemical marker in the future breeding programmes. The rust resistance sources do not possess γ-45/ Gli-1 alleles, so these lines can be used as donors to introduce disease resistance in the good quality recently released varieties, which are containing γ-45/ Gli-1 alleles. From hierarchical analysis, it was found that landraces, released varieties and rust resistance sources are genetically distinct. The presence of new γ-gliadin patterns are interesting in rust resistance sources and need to be investigated for their role in pasta making as well as overall technological quality of durum wheat.

Restricted access

Function. Conference Proceedings 2001 Gregova, E., Mihalik, D., Slikova, S., Sramkova, Z. 2007. Allelic variation of HMW

Restricted access

. Gupta, R.B., MacRitchie, F. 1994. Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3 and Gli-1 of common wheats. II. Biochemical basis of the allelic effects on dough properties. J. Cereal Sci. 19 :19

Restricted access

. 1985 70 634 642 Gupta, R.B., MacRitchie, F. 1994. Allelic variation at glutenin subunit and gliadin loci, Glu-1, Glu-3

Restricted access

89 95 Liu, L., He, Z., Yan, J., Zhang, Y., Xia, X., Pena, R.J. 2005. Allelic variation at the Glu-1 and Glu-3 loci, presence of the 1B.1R translocation, and their effects on

Restricted access

High molecular weight glutenin and 1BL.1RS translocation employing the standard sodium dodecyl sulphate (SDS-PAGE) and acid (A-PAGE) polyacrylamide gel electrophoresis methods were classified in 43 Slovak wheat cultivars registered between 1976 and 2006. Total number of 9 alleles was detected at all Glu-1 loci. The most frequent HMW-GS alleles were “Null” for Glu-1A , 7+9 for Glu-1B and 5+10 for Glu-1D , respectively. At the same time these alleles also constituted the most frequent HMW-GS genotype and phenotype-0, 7+9, 5+10. Such HMW-GS combination was found in 48.8% of all genotypes analyzed in Triticum aestivum L. ssp. aestivum . Eleven different HMW-GS genotype-phenotype combinations were found, occurring at various frequencies.

Restricted access

Variation at the Xgwm261 locus was studied in 92 Indian bread wheat varieties. The locus is of interest because its 192 bp allele is reported to be linked to semi-dwarfing gene Rht8. There were five allelic variants based on size. Occurrence of 165, 192 and 174 bp alleles was 44.5, 39.1 and 10.8%, respectively. Sequencing showed six new alleles. Cvs. C306 and PBW516 showed 192 bp alleles which were different from each other, different from other varieties and also from reported sequence. A 200 bp band in four varieties was unique and different in CT repeats and sequence motif next to the repeat region. Cv. NI179 showed new 198 bp allele. The varieties were also analysed with allele specific primers for Norin10 genes. Among semi-dwarf varieties, frequencies of RhtB1b and RhtD1b were 48.5 and 45.7%, respectively. The relation between alleles of Xgwm261 and agronomic traits was studied by growing plants at two locations. The 192 bp allele did not show association with grain yield or its components at Trombay location. Differences in plant height among the 192, 165 and 174 bp allele carriers were not significant at both the locations indicating that the 192 bp allele in Indian varieties was neutral with respect to plant height. Frequency of 165 bp allele was higher in varieties recommended for warmer zones and late sown conditions.

Restricted access
Biologia Futura
Authors: Zoltán Attila Köbölkuti, Klára Cseke, Attila Benke, Mátyás Báder, Attila Borovics, and Róbert Németh

Introduction

Since Populus has veritable value as timber, plywood, pulp, and paper, genomic research should create the sound basis for further breeding toward desirable wood quality attributes.

Materials and methods

In this study, we addressed the need for a research methodology that initially identifies and then characterize candidate genes encoding enzymes with wood property phenotypic traits, toward the aim of developing a genomics-based breeding technology.

Results

On 23 different poplar species/hybrid samples, we successfully amplified 55 primers designed on Populus trichocarpa L. Considering the number of polymorphic sites, out of 73,206 bp, 51 SNPs and 31 indel events were found. Non-synonymous single base mutations could be detected in number of 30, 21 out of 164 sequences were the number of minimum recombination events and 41 significant pairwise comparisons between loci could be detected.

Discussion and conclusion

Our results provide a roadmap for a future association genetic study between nucleotide diversity and precise evaluation of phenotype.

Open access