Authors:Á. Halász, E. Horváth-Szanics, M. Nagy-Gasztonyi, J. Pauk and Gy. Hajós
Protein sets, enzyme activities and immune reactivity against wheat germ agglutinin in the albumin-globulin fractions of parent and herbicide resistant transgenic wheat lines were studied.Our results showed significantly increased amylase activities and increased immune reactivity against wheat germ agglutinin in the herbicide resistant transgenic wheat lines, investigated. The amylases and lectins belong to the plant food allergens; this explains why both scientists and consumers are interested in assessing the allergenic potential of plant proteins and the safety assessment of novel foods and GM foods in highlight of food safety. This paper is an important contribution to our database and the understanding of what is going on with genetic engineering of crop plants.
Authors:K Baintner, P Kiss, S Bardocz and A Pusztai
Short-term effects of orally administered plant lectins, with special reference to the Phaseolus vulgaris agglutinin (phytohaemagglutinin, PHA), were studied in growing rats. The orally administered PHA elicited a dose-dependent accumulation of liquor with elevated pH in the proximal small intestine. Although the concentration of a-amylase activity did not change, total a-amylase activity slightly, but significantly increased in the gut. When a panel of plant lectins with different carbohydrate binding specificities was tested at the dose of 100 mg/kg body weight, most of them stimulated the secretion of liquor, but the total a-amylase activity was increased only by PHA, ConA or WGA.
Delcour, J.A., Verschaeve, S.G. 1987. Malt diastatic activity. Part II. A modified EBC diastatic power assay for the selective estimation of beta-amylaseactivity, time and temperature dependence of the release of reducing sugars. J. Inst. Brew.
Authors:A. Fistes, D. Soronja Simovic, D. Rakic and J. Mastilovic
Number” according to Hagbergas a measure of the degree of alpha-amylaseactivity in grain and flour . International Association for Cereal Science and Technology. No. 107/1. pp. 1–22.
ICC Standard (1995): Determination of
Durusoy, M., Tipirdamaz, R. and Bozcuk, S. (1995): Effect of exogenously applied spermidine and gibberellic acid on alpha-amylaseactivity of germinating barley seeds under salinity stress. - Turkish J. Biol . 19 : 111