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Cai Q., Szarejko I., Polok K., Maluszyński M. (1992). The effect of sugars and growth regulators on embryoid formation and plant regeneration from barley anther culture. Plant Breeding 109: 218

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., Walden, R.M. 1991. Plastid genome structure and plastid-related levels in albino barley plants derived from anther culture. Curr. Genet. 20 :339–347. Walden R.M. Plastid genome

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., Geng, G. (1989): A new synthetic medium (W 14 ) for wheat anther culture. Annu. Rep. Genet. Sin. , Beijing, pp. 91-92. Jiménez, V. M. (2001): Regulation of in vitro somatic embryogenesis with emphasis on the role

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Wu, L. Y., Kiang, T. (1979): Using isozyme marker to detect pollen derived plant from anther culture of wild rice. Bot. Bull. Sinica , 20, 97-102. Using isozyme marker to detect pollen

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The objective of this study was to develop a simple anther culture protocol for a range of Saudi wheat genotypes. Seven wheat genotypes were evaluated in anther culture on five different medium protocols for their ability to initiate callus and green plants. The estimates of significance for the effects of genotypes, and medium protocols used, and their interactions on callus induction, callus weight and shoot formation derived from anther explants indicated that the in vitro traits were significantly influenced by the genotypes, medium protocols, and their interactions. The percentage of explants that developed calli ranged from 0.41% (Lang) to 15.39% (Irena) averaged across the five medium protocols with an average 4.45%. The genotype Irena produced the highest average means of shoot formation (69.65%) across medium protocols. The genotype Yecora Rojo (13.73%) was significantly inferior to all other tested genotypes for shoot formation.

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Bullock W.P., Beamier P.S., Schaeffer G.W., Bottino P.S. 1982. Anther culture of wheat ( Triticum aestivum L.) F 1 ’s and their reciprocal crosses. Theor. Appl. Genet. 59:333–340 Bottino P

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Belchev, I., Kostov, K., Schlegel, R., Ivanov, P., Tsenov, N., Stavreva, N. 2000. Anther culture response of wheat ( Triticum aestivum L.) varieties from Eastern Europe. Bulg. J. Agr. Sci. 6 :499

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Armstrong, T. A., Metz, S. G., Mascia, P. N. (1987) Two regeneration systems for the production of haploid plants from wheat anther culture. Plant Sci. 51 , 231-237. Two regeneration

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.C., Wang, C.C., Sun, C.S., Hsu, C., Yin, K.C., Chu, C.Y., Bi, F.Y. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci. Sin. 18 :659

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Acta Agronomica Hungarica
Authors: B. Barnabás, T. Spitkó, K. Jäger, J. Pintér and L. C. Marton

Barloy, D., Denis, L., Beckert, M. (1989): Androgenic aptitude of DH lines obtained through anther culture. Maydica , 34 , 303-308. Androgenic aptitude of DH lines obtained through

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