In the present work the effect of microalgal and cyanobacterial biomass on anther cultures of wheat (Triticum aestivum L.) was studied. Investigations were made on the influence of media supplemented with a biomass of four terrestrial and fresh-water microalgal and cyanobacterial strains (1-2 g l-1) on the androgenic response, frequency of microspore-derived embryo-like structures, and their regeneration capacity in anther cultures of wheat. The addition of 1 g L-1 of cyanobacterium biomass MACC 643 to the induction and regeneration media significantly improved the androgenic response by 50% in the genotype Mv Pálma, and reduced the requirement for the synthetic auxin 2,4-D.
Authors:M. Barakat, A. Al-Doss, A. Elshafei, K. Moustafa, and E. Ahmed
The objective of this study was to develop a simple anther culture protocol for a range of Saudi wheat genotypes. Seven wheat genotypes were evaluated in anther culture on five different medium protocols for their ability to initiate callus and green plants. The estimates of significance for the effects of genotypes, and medium protocols used, and their interactions on callus induction, callus weight and shoot formation derived from anther explants indicated that the in vitro traits were significantly influenced by the genotypes, medium protocols, and their interactions. The percentage of explants that developed calli ranged from 0.41% (Lang) to 15.39% (Irena) averaged across the five medium protocols with an average 4.45%. The genotype Irena produced the highest average means of shoot formation (69.65%) across medium protocols. The genotype Yecora Rojo (13.73%) was significantly inferior to all other tested genotypes for shoot formation.
Authors:G. Ganeva, S. Landjeva, I. Belchev, and L. Koleva
Belchev, I., Kostov, K., Schlegel, R., Ivanov, P., Tsenov, N., Stavreva, N. 2000. Anthercultureresponse of wheat ( Triticum aestivum L.) varieties from Eastern Europe. Bulg. J. Agric. Sci. 6 :499–506.