Authors:A Smetanka, V Stara, I Farsky, I Tonhajzerova, and I Ondrejka
, perturbations in appetite and sexual desire, constipation, crying, and suicidal thoughts ( 5 ). The pharmacotherapy of MDD consists of the use of antidepressants, which operate on the central neurotransmitter system and modulate its action ( 19 ). Antidepressant
Authors:Cody Sykes Gilbert, Mitch Earleywine, Maha N. Mian, and Brianna R. Altman
). Psychotherapy tends to include candid discussions designed to alter relevant thinking processes and goals in an effort to increase activities consistent with a client's values ( Cuijpers et al., 2020 ). Available treatments have serious drawbacks. Antidepressant
Authors:Ł. P. Haliński, D. Śmigiel, M. Czerwicka, M. Paszkiewicz, J. Kumirska, and P. Stepnowski
The evaluation of several derivatization procedures for the gas chromatographic analysis of selected anticancer pharmaceuticals (cyclophosphamide, iphosphamide, flutamide, chlorambucil, and melphalan) in the presence of tricyclic antidepressants was carried out. Among the methods, concerning trimethylsilylation, tert-butyldimethylsilylation, and methylation, the most useful was methylation using (trimethylsilyl)diazomethane (TMSD), which is a safe alternative for the common reaction with diazomethane. Most of the anticancer drugs were unstable during derivatization using silylating agents, while antidepressants were stable in all tested conditions. Melphalan was the only compound, for which the results of all tested procedures were not satisfying. The TMSD-based procedure was validated, giving the results possibly suitable for the screening purposes in contaminated environmental samples.
The selectivity of the TLC separation of six new antidepressant substances has been investigated on silanized silica gel C
and on silica gel GF
. Optimization of the retention and selectivity of these compounds on reversed-phase plates (RP C
) was performed by changing the pH and the concentration of organic solvent (methanol, acetonitrile, tetrahydrofuran) in the aqueous mobile phases. The substances were separated in horizontal chambers and the drugs were detected by use of a videoscanning system and illumination of the plates at
= 254 nm. The drugs were also separated on silica gel GF
after solid phase extraction (SPE) from plasma. The best separation was achieved with benzene-acetone-ethanol-ammonia, 9 + 7 + 2 + 1 (
) as mobile phase.
Thyroid hormones (TH) are supposed to control the activity of some neurotransmitters (e.g., serotonin), which are hypothetically
involved in the pathogenesis of depressive illness. A new group of non-tricyclic antidepressant drugs includes selective serotonine
re-uptake inhibitors. The most frequently used representative of this group is fluoxetine (Fluox). We followed in the present
paper the effects of Fluox, administered subchronicaly (for 25 days) to Wistar rats by itself, or in combination with 3,3′,5-triiodo-l-thyronine (T3). In studies of the interaction of Fluox with the metabolism of TH, we applied adapted radiometric enzyme assays for iodothyronine
sulfotransferases (ST) and uridine 5′-diphospho-glucuronyltransferase (UDP-GT), as well as our newly developed radiometric
assays for iodothyronine deiodinases (IDs) of types 1, 2 and 3 (D1, D2 and D3), using 125I-labelled iodothyronines of high specific radioactivity as substrates. We found about two-fold higher UDP-GT enzyme activities
in samples of liver microsomes of rats treated with Fluox, in comparison with control rats. In contrast, the radiometric determination
of ST activities in liver and kidney cytosolic fractions did not demonstrate any significant effects of the administration
of Fluox, alone or together with T3, on the induction of these enzymes. However, profound changes in enzyme activities were determined in case of IDs, especially
in the pituitary and cerebellum of treated rats. The adapted and newly elaborated radiometric enzyme assays proved to be very
sensitive and rapid and, at the same time, reliable and robust.