Search Results

You are looking at 1 - 10 of 25 items for :

  • "beta amyloid" x
  • All content x
Clear All

The aim of this study was to investigate the effects of maternal lead exposure on the learning and memory ability and expression of tau protein phosphorylation (P-tau) and beta amyloid protein (Aβ) in hippocampus of mice offspring. Pb exposure initiated from beginning of gestation to weaning. Pb acetate administered in drinking solutions was dissolved in distilled deionized water at the concentrations of 0.1%, 0.5% and 1% groups. On the 21 th of postnatal day, the learning and memory ability of the mouse pups was tested by Water Maze test and the Pb levels in blood and hippocampus of the offspring were also determined. The expression of P-tau and Aβ in hippocampus was measured by immunohistochemistry and Western blotting. The Pb levels in blood and hippocampus of all exposure groups were significantly higher than that of the control group ( P < 0.05). In Water Maze test, the performances of 0.5% and 1% groups were worse than that of the control group ( P < 0.05). The expression of P-tau and Aβ was increased in Pb exposed groups than that of the control group ( P < 0.05). Tau hyper-phosphorylation and Aβ increase in the hippocampus of pups may contribute to the impairment of learning and memory associated with maternal Pb exposure.

Restricted access

Alzheimer's disease (AD) related beta amyloid (Aβ) peptides possess high propensity towards aggregation. Their diffusion-controlled association follows a physico-chemically well-defined kinetics: the fibrillization starts from the monomeric/ dimeric state, and proceeds in the direction of oligomeric→protofibrillar→ fibrillar state producing neurotoxic aggregates. Nowadays one of the major directions of the drug design against AD is the synthesis of putative amyloid aggregation inhibitor molecules (AAI) which are able to hinder the formation of these toxic amyloid aggregates. Studies of both the Aβ aggregation and the effect of the AAIs on this process can be performed with several instrumental techniques. The size distribution of the aggregates up to the micron size range can be characterized with dynamic light scattering (DLS). On the other hand, species having a diameter above 5 nm can be visualized with transmission electron microscopy (TEM). In this work, we propose standardized sample preparation protocols in order to gain a reproducible aggregation profile of the Aβ peptides according to the experimental requirements. Besides, we investigate the effect of our formerly designed AAI, the RIIGLa pentapeptide on the aggregation of Aβ[1-42]. Based on our DLS and TEM results, we demonstrate the aggregation altering ability of this pentapeptide.

Restricted access
European Journal of Microbiology and Immunology
Authors: Christina Fröhlich, Kristin Paarmann, Johannes Steffen, Jan Stenzel, Markus Krohn, Hans-Jochen Heinze, and Jens Pahnke

396 405 . 15. P.E. Cramer 2012 ApoE-directed therapeutics rapidly clear beta-amyloid and reverse deficits in AD

Restricted access

Az inclusiós testes myositis az idiopathiás inflammatorikus myopathiák vakuólaképződéssel járó formái közé tartozik, azok 15–28%-át képezi. Fontos a korai felismerés, mert alattomosan progrediáló betegségről van szó, és a már kialakult izomatrófia nehezen kezelhető. Ötven év felett a leggyakoribb krónikus progresszív izombetegség. Patomechanizmusában egyrészt degeneratív folyamatok, a béta-amyloid-lerakódás játszik szerepet, másrészt a polymyositishez hasonlóan autoinvazív klonális CD8+-T-lymphocyták által mediált celluláris citotoxicitás. A két eltérő patomechanizmust jól szemlélteti a biopsziás mintákban jelen lévő vakuolizált izomrostok, illetve a gyulladásos sejtek által körülvett, nem vakuolizált izomrostok párhuzamos jelenléte. Az MHC-I/CD8 komplex mint specifikus immunmarker bevezetésre került az újonnan kidolgozott diagnosztikus kritériumrendszerben, ez segít elkülöníteni az egyéb izomdisztrófiákban észlelhető aspecifikus gyulladástól. Klinikailag a distalis és proximális izmok érintettsége, légzőizom-gyengeség, dysphagia jellemzi, interstitialis tüdőbetegség nem jellemző. Általában terápiarezisztens, ennek magyarázata lehet a degeneratív folyamatok túlsúlya. Emiatt kortikoszteroid- és egyéb immunszuppresszív terápia sok esetben csak biokémiai választ eredményez, amelyet klinikai válasz nem kísér. A betegség diagnózisa, illetve differenciáldiagnózisa különösen nagy kihívást jelent a gyakorló klinikus számára, tekintettel a specifikus immunhisztokémiai hátteret igénylő diagnosztikus nehézségekre. Az újabb terápiás támadáspontok, kostimulatorikus molekulák elleni antitestek, anticitokin-terápia alkalmazása további előrelépést nyújthat a betegek életminőségének javításában.

Restricted access

. 79 569 572 Yasojima, K., McGeer, E. G., McGeer, P. L. (2001) Relationship between beta amyloid peptide generating molecules and neprilysin in

Restricted access

Hickman SE et al.: Microglial dysfunction and defective beta-amyloid clearance pathways in aging Alzheimer’s disease mice. J Neurosci 28(33), 8354–8360 (2008) Hickman S.E. Microglial

Open access
Orvosi Hetilap
Authors: Gyöngyvér Lacza and Zsolt Radák

in preventing high fat diet-induced beta-amyloid deposition and memory deficit in amyloid precursor protein transgenic mice. J. Biol. Chem., 2012, 287 , 23024–23033. Kubota M

Open access
Acta Veterinaria Hungarica
Authors: Adriana Bravo-Monsalvo, Juan Vázquez-Chagoyán, Lilia Gutiérrez, and Héctor Sumano

Lecanu, L., Wenguo, Y., Xu, J., Greeson, J. and Papadopoulos, V. (2005): Local anesthetic procaine protects rat pheochromocytoma PC12 cells against beta-amyloid-induced neurotoxicity. Pharmacology 74 , 65

Restricted access

Li, N., Yu, Z. L., Wang, L., Zheng, Y. T., Jia, J. X., Wang, Q., Zhu, M. J., Liu, X. L., Xia, X., Li, W. J. (2010) Increased tau phosphorylation and beta amyloid in the hippocampus of mouse pups by early life lead exposure. Acta Biol. Hung. 61 , 123

Restricted access
Acta Biologica Hungarica
Authors: Márta Kotormán, Zita Kelemen, Phanindra Babu Kasi, and János Nemcsók

) Melissa officinalis acidic fraction protects cultured cerebellar granule neurons against beta amyloid-induced apoptosis and oxidative stress . Cell J. 18 , 556 – 564 . 34. Soto

Restricted access