Authors:Qinyi Fu, Hongwei Zheng, Xiangning Han, Limin Cao, and Jianxin Sui
Introduction Biogenicamines, which are a general term for a class of biologically active nitrogen-containing organic compounds with low molecular weight, have been found in various foods such as cheese, wine, meat and aquatic products [ 1 ]. It has
Csaba , G. , Kovács , P. , Pállinger , É. : Edac fixation increases the demonstrability of biogenicamines in the unicellular Tetrahymena: A flow cytometric and confocal microscopic comparative analysis . Cell Bol Int 30 , 345 – 348 ( 2006
Authors:D. Giuffrida, M. Ziino, A. Verzera, C. Condurso, and V. Romeo
The biogenic amine
content of “Provola dei Nebrodi”, a typical “pasta filata” Sicilian cheese, was
studied at different ripening stages. Reversed-phase high performance liquid
chromatography (RP-HPLC) with fluorimetric detection was employed for the identification
and quantification of eight different amines: ethanolamine, histamine,
serotonine, tyramine, tryptamine, 2-phenylethylamine, putrescine and
cadaverine. Histamine was the most representative amine in all the analysed
cheese samples, followed by serotonin and tyramine. The total amount of
biogenic amines was lower than 0.1 ppm in fresh samples, whereas it reached
33.57 ppm in samples with the longest ripening time. The biogenic amine
contents were correlated with the proteolytic maturation coefficient, defined
as the water-soluble nitrogen/total nitrogen (WSN/TN %) percent ratio. The
results showed amine contents lower than the toxic level even for samples with
the longest ripening time.
Stratton, J. E., Hutkins, R. W. & Taylor, S. L. (1991): Biogenicamines in cheese and other fermented foods: A review. J. Fd Prot , 54 , 460-470.
Biogenicamines in cheese and other fermented foods: A review
values and mobile-phase composition have been determined for six methylxanthines and acidic and neutral biogenic amine metabolites on silica gel with mobile phases comprising a non-polar or weakly polar diluent (chloroform, heptane, or hexane) and a polar modifier (THF, dioxane, acetone, or ethyl acetate). The relationships obtained constitute a retention database providing useful information for TLC sample-screening methods and for isolation and preconcentration of the compounds of interest by solid-phase extraction (SPE) on silica columns.
The dependence on mobile-phase pH of the retention behavior of biogenic amine neurotransmitters and their metabolites in reversed-phase thin layer chromatography has been investigated. Chromatography was performed on RP-18 with mixtures of buffers of different pH (2.00, 3.00, 4.00, 5.00) and methanol, in different proportions (5–40% MeOH) as mobile phases. Relationships between retention (
) and mobile-phase composition for these compounds form a database which can be used to plan selective and rugged HPLC separations of these compounds.
The possibility of using molecular descriptors to predict both the retention behavior and the mechanism of retention of biogenic amine neurotransmitters and some of their metabolites in TLC has been examined. QSRR analysis was achieved by simultaneous solution of a set of linear equations. Basic correlations were found between retention and molecular descriptors calculated by means of semi-empirical methods of quantum chemistry. The functions obtained provided insight into the mechanism of chromatographic retention on a molecular level. Experimentally obtained retention data for biogenic amine neurotransmitters and their derivatives also served as a basis for cluster analysis of these TLC systems.