Authors:Csaba Jakab, Miklós Rusvai, Péter Gálfi, Ágnes Szabára, Zoltán Szabó, and Janina Kulka
Claudins are key tight junctional proteins between adjacent epithelial, mesothelial or endothelial cells, which are responsible for the permeability of the paracellular space. This paper describes that the endothelial cells of normal hepatic arterioles, portal venules and portal lymphatics as well as the endothelium of sinusoids from dogs show strong membranous claudin-5 cross-reactivity. In 25 liver biopsy samples taken from dogs with portal vein hypoperfusion, an increased number of arterioles was detected in the portal areas (PAs) by the use of humanised anti-claudin-5 antibody. The increased number of hyperplastic hepatic arterioles per PA was 5–6, 8–12 and 15–20 in the case of small, medium-sized and large PAs, respectively. It is suggested that the claudin-5 marker can improve the detection of hepatic arteriolar proliferation in the PAs of liver samples.
Authors:Godwins Okagbo Echejoh, Matthew N. Tanko, Agabus N. Manasseh, Stella Ogala-Echejoh, Barnabas Mandong, and Edith Okeke
biopsy and microbiological tests inpatients with HIV infection Presse Med. 25 1147 – 1151 .
. P. Escartin Munoz-Ortiz J. A. Lopez G-Asenjo C. Garcia Gonzalez V. Roca Arbones C. Taxonera Samso R. Pieltain Alvarez
Authors:Zoltán Mátrai, Ferenc Bánhidy, Melinda Téglás, Eszter Kovács, Ákos Sávolt, Nóra Udvarhelyi, Alexandra Bartal, and Miklós Kásler
Spanheimer, P. M., Graham, M. M., Sugg, S. L., et al.: Measurement of uterine radiation exposure from lymphoscintigraphy indicates safety of sentinel lymph node biopsy during pregnancy. Ann. Surg. Oncol., 2009, 16 , 1143
Authors:B. Baranyai, Sz. Bodó, A. Dinnyés, and Elen Gócza
Solid surface vitrification (SSV) was compared with in-straw vitrification for cryopreservation of biopsied mouse embryos. Eight-cell stage embryos were zona drilled and one blastomere was removed. Developed morulae or blastocysts were vitrified in microdrop (35% EG + 5% PVP + 0.4 M trehalose) or in straw (7.0 M EG + 0.5 M sucrose). Following recovery, embryos were cultivated in vi tro or transferred into recipients. Cryopreservation had an effect not only on the survival of biopsied embryos but also on their subsequent development in vitro. Cryosurvival of biopsied morulae vitrified in straw was significantly inferior to SSV. The post-warm development of biopsied and non-biopsied morulae was delayed on Day 3.5 and 4.5 in both vitrification groups. A delay in development was observed on Day 5.5 among vitrified non-biopsied blastocysts. The percentage of pups born from biopsied morulae or blastocysts following cryopreservation did not differ from that of the control. No significant differences could be detected between methods within and between embryonic stages in terms of birth rate. The birth rate of biopsied embryos vitrified in straw was significantly lower compared to the non-biopsied embryos. The novel cryopreservation protocol of SSV proved to be effective for cryopreservation of morula- and blastocyst-stage biopsied embryos.