milk. Many other factors, such as maternal age, term of delivery, stage of lactation, and gestational diseases, may influence the FA composition of milk ( Argov-Argaman et al., 2016 ). Breastmilk fatty acid profile and TAGs content changes have been
detection of the studied drugs in breastmilk was determined. PAR passes into milk in greater quantities compared to CQ and ASP as the protein binding of PAR appears at concentration greater than 60 µg/mL with percentage ranging from 15 to 21% [ 10 ]. While
A new, sensitive, and selective high-performance liquid chromatography (HPLC) method with fluorimetric detection was developed for the determination of moxifloxacin (MOX) in human breast milk. MOX was precolumn derivatized with fluorescamine; the fluorescent derivative was separated on an RP C18 column using a mobile phase composed of acetonitrile–10 mM orthophosphoric acid by isocratic elution with flow rate of 0.5 mL min−1. The method was based on the measurement of the derivative using fluorescence detection at 481 nm with excitation at 351 nm. The calibration curve was linear over the range of 1–40 μg mL−1. Limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.3 and 1 μg mL−1, respectively. Intra-day and interday repeatabilities were less than 3.15%.
In this study, we examined the relationship between levels of lactoferrin (LF) and IL-17 in human serum and breast milk and the development of allergy in children. LF and IL-17 levels were determined by ELISA in healthy (n=19) and allergic mothers (n=21) on the 5th day after delivery. Two years later, information on breastfeeding and allergic outcomes was collected by questionnaires from parents of both groups and district child care nurses. Significantly higher concentrations of LF were found in the breast milk of allergic mothers compared to the healthy controls. At 2 years of age, only those three infants became allergic from the atopic group in whose starting breast milk samples a very high LF level (306 μg mg–1 protein) or simultaneously elevated concentrations of LF and IL-17 were measured. These findings indicate that the very early measurement of LF and IL-17 levels in the breast milk of allergic mothers may help to predict the allergy development in their infants.
1 Introduction Many recent studies have shown that there are inherent differences in taste between human breastmilk and infant formula composition, which opens unresolved questions regarding implications of this notion on dietary habits, food
habits, geographic region, obesity ( Butts et al., 2018 ). Lindholm et al. (2013) found that obese mothers' milk contained fewer n-3 fatty acids than normal weight mothers' milk. This resulted in a 40% higher n-6/n-3 ratio in breastmilk from obese
Instrumental neutron activation analysis (INAA) and proton induced X-ray emission (PIXE) analysis were used to determine the concentration of 13 elements in the breast milk collected within the first week of lactation and after morning feed from 16 pre-term mothers and 20 term mothers. The results of the study show that pre-term milk has a significantly higher concentration of Cl, Cu, Fe, K, Mg, Mn, Na and Zn while term mothers have a significantly higher concentration of P and Br. No significant differences were, however, found in the mean concentration of Al, Ca, I and Rb. Furthermore, the mean concentration for most of the elements found in the breast milk fall within the range of the concentrations obtained for the commercial infant milk formulae.
The influence of stages of lactation on the mineral and trace elementconcentrations of breast milk collected from 20 healthy lactating women ina Nigeria population was investigated using instrumental neutron activationanalysis (INAA) and proton induced X-ray emission (PIXE) analysis. The breastmilk samples were divided into colostrum (day 1–5), transitional (day6–13) and mature milk (day 14–28). The result of the study showthat colostrum milk has a significantly higher mean concentration of Ca, Cl,Cu, Fe, Mg, Na and Zn than the transitional and mature milk. Furthermore,the mean concentrations of Fe, Mg, Na and Zn in transitional milk were foundto be significantly higher when compared with the mature milk. It was alsoobserved from the semi-log plot of the concentration of the elements againstdays of lactation that the concentration of all the elements decreased significantlythroughout the lactation period studied with Cu, Fe and Zn concentration showingthe greatest decline, particularly in the early stages of lactation.
source of probiotic lactic acid bacteria (LAB). The most commonly found and isolated bacteria species from human breastmilk include Lactobacillus , Bifidobacterium , Streptococcus , and Enterococcus . Human milk bacteria are gaining much attention