, pmol/µg for 3-NT, pg/µg for TRPV1, and ng/µ for CGRP. Measurements of Ca 2+ levels in the colon A Colorimetric Calcium Detection Assay Kit (Abcam, ab102505) was used to determine calcium (Ca 2+ ) levels in the colon. Samples were homogenized on ice
A simple graphical linear
method was introduced for isothermal titration calorimetric data analysis
in the protein-ligand interaction. The number of binding sites, the dissociation
binding constant and the molar enthalpy of binding site can be obtained by
using this new isothermal titration calorimetric data analysis method. The
method was applied to the study of the interaction of human growth hormone
(hGH) with divalent calcium ion at 27C in NaCl solution, 50 mM. hGH has
a set of three identical and independent binding sites for Ca 2+
. The intrinsic dissociation equilibrium constant and the molar enthalpy of
binding are 52 μMand -17.4, respectively. Results obtained by this
new calorimetric data analysis are in good agreement with results obtained
using our previous method.
The most widely identified degradation process suffered by calcium aluminate cement (CAC) is the so-called conversion of hexagonal
calcium aluminate hydrate to cubic form. This conversion is usually followed by an increase in porosity determined by the
different densities of these hydrates and the subsequent loss of strength. Mixes of calcium aluminate cement (CAC) and silica
fume (SF) or fly ash (FA) represent an interesting alternative for the stabilization of CAC hydrates, which might be attributed
to a microstructure based mainly on aluminosilicates. This paper deals with the microstructure of cement pastes fabricated
with mixtures CAC-SF and CAC-FA and its evolution over time. Thermal analysis (DTA/TG), X-ray diffraction (XRD) and mid-infrared
spectroscopy (FTIR) have been used to assess the microstructure of these formulations.
Rhizosphere and rhizoplane of fababean (Vicia faba), melochia (Corchorus olitorius), sesame (Sesamum indicum) and soyabean (Glycine max) plants are inhabited with fungi, mostly Aspergillus flavus, A. niger, Fusarium oxysporum, Penicillium corylophilum, P. cyclopium, P. funiculosum and Rhizopus stolonifer. All fungal species have the ability to produce gibberellin (GA) but F. oxysporum was found to produce both GA and indole-acetic acid (IAA). The optimum period for GA and IAA production by F. oxysporum was 10 days in the mycelium and 15 days in the filtrate at 28 °C. The contents of GA, IAA and cytochrome P-450 were increased at 0.5 and 1% NaCl after 5 days, but GA and IAA were lowered at 4% (700 mM) NaCl. Calcium decreased NaCl stress on F. oxysporum by significant elevating GA biosynthesis at 40mM Ca2+/700 mM Na+. GA at 10 mM and Ca2+ at 10 mM enhanced the germination of seeds under 175 mM Na+.
Calcium aluminate cement (CAC) is known as an indispensable material in the construction field for its resistance to chemical attack and high temperatures [ 1 ]. In the dry-mix mortar industry, such as self