Authors:J. Chen, G. Hu, J. Zhang, C. Chu and Y. Wu
Drought is a severe abiotic stress that affects wheat production worldwide. In order to identify candidate genes for tolerance to water stress in wheat, sequences of 11 genes that have function of drought tolerance in other plant species were used to identify the wheat ortholog genes via homology searching in the wheat EST database. Atotal of 11 primer pairs were identified and amplified PCR products in wheat. Of them, 10 STS markers were mapped on 11 chromosomes in a set of nulli-tetrasomic lines of ‘Chinese Spring’ wheat; six were mapped on chromosomes 1A, 1B, 4B, 7A, 2B and 5D, respectively, in a spring wheat mapping population (POP1). The marker XTaABH1 mapped on 7A in POP1 was the only one mapped but characterized in a winter wheat mapping population (POP2) for grain yield, kernel weight and diameter, and height in four-field trials applied different water stress or irrigation. The marker XTaABH1 was significantly associated with grain yield under rainfed condition, with kernel weight under terminal stress and non-irrigation conditions, with kernel diameter and height under non-irrigated condition. The STS primers, map information and marker-trait association produced in the currently study would be of interest to researchers working on drought tolerance.
Authors:Zoltán Attila Köbölkuti, Klára Cseke, Attila Benke, Mátyás Báder, Attila Borovics and Róbert Németh
promising – clones cultivated in Hungary.
Our first goal was to identify SNP markers within candidategenes encoding cellulose and lignin biosynthetic enzymes, with an assumed role in wood property phenotypic traits. All candidate proteins have been
Authors:Y.Q. Wang, X.J. Hou, B. Zhang, W.J. Chen, D.C. Liu, B.L. Liu and H.G. Zhang
Red coleoptile is an easily observed agronomic trait of wheat and has been extensively studied. However, the molecular mechanism of this trait has not yet been revealed. In this study, the MYB gene TaMYB-D1 was isolated from the wheat cultivar ‘Gy115’, which possesses red coleoptiles. This gene resided at the short arm of the homoelogous group 7 chromosomes. TaMYB-D1 was the only gene expressed in the coleoptiles of ‘Gy115’ and was not expressed in ‘Opata’ and ‘CS’, which have uncoloured coleoptiles. Phylogenetic analysis placed TaMYB-D1 very close to ZmC1 and other MYB proteins regulating anthocyanin biosynthesis. The encoded protein of TaMYB-D1 had an integrated DNA binding domain of 102 amino acids and a transcription domain with 42 amino acids, similar to the structure of ZmC1. Transient expression analysis in onion epidermal cells showed that TaMYB-D1 was located at the plant nucleus, which suggested its role as a transcription factor. The expression of TaMYB-D1 was accompanied with the expression of TaDFR and anthocyanin biosynthesis in the development of the coleoptile of ‘Gy115’. Transient expression analysis showed that only TaMYB-D1 induced a few ‘Opata’ coleoptile cells to synthesize anthocyanin in light, and the gene also induced a colour change to red in many cells with the help of ZmR. All of these results suggested TaMYB-D1 as the candidate gene for the red coleoptile trait of ‘Gy115’.
Authors:Á. Baráth, E. Endreffy, Cs. Bereczki, B. Gellén, B. Szűcs, I. Németh and S. Túri
Halushka MK, Fan JB, Bentley K, Hsie L, Shen N, Weder A, Cooper R, Lipshutz R, Chakravarti A: Patterns of single-nucleotide polymorphisms in candidategenes for blood-pressure homeostasis. Nat. Genet. 22(3), 239–247 (1999
Authors:Gaigai Du, Liyuan Wang, Huawei Li, Peng Sun, Jianmin Fu, Yujing Suo, Weijuan Han, Songfeng Diao, Yini Mai and Fangdong Li
To determine the expression profiles of candidategenes from different tissues, we collected the roots, stems, leaves, female floral buds, fruits, and seeds of 10-year-old monoecious D. kaki “Zenjimaru.” To determine the
, J. , Kereszturi , É. , Gádoros , J. , Sasvári-Székely , M. , Nemoda , Zs. ( 2007 ): Dopaminergic CandidateGenes in Tourette Syndrome: Association Between Tic Severity and 3’ UTR Polymorphism of the Dopamine Transporter Gene . American
Handa, H., Namiki, N, Xu, D.H, Ban, T. 2008. Dissecting of the FHB resistance QTL on the short arm of wheat chromosome 2D using a comparative genomic approach: from QTL to candidategene. Molecular Breeding