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Authors:A. K. Tewari, A. N. Mukhopadhyay and R. Aggarwal
Chickpea (Cicer arietinum) seeds treated with powdered preparation of Gliocladium virens (Gv) alone @ 3 g/kg seed or in combination with vitavax (0.1%) showed colonization of G. virens on seed coat, collar region, plumule and radicle. Microscopic examination revealed that colonization of seed with mycelia and spores of antagonist started 24 h after incubation. Major portion of the seedling was covered with in 48 hrs. Population dynamics of G. virens monitored at different time intervals in spermosphere, rhizosphere and non-rhizosphere region in pathogen infested and non-infested soil using Trichoderma Selective Medium showed that population of G. virens increased initially up to 30 days and then gradually declined. The highest population was observed in spermosphere (7 x 105/g) followed by rhizosphere (6.3 x 104/g), when seeds treated with Gv + vitavax were sown in pathogen infested soil.