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Acta Chromatographica
Authors:
Lenche Velkoska-Markovska
,
Mirjana S. Jankulovska
,
Biljana Petanovska-Ilievska
, and
Kristijan Hristovski

chlorogenic acid, are known to influence coffee flavor, acidity, and bitterness [ 3 ]. Chlorogenic acid and its derivatives are few of the main phenolic compounds present in coffee [ 4 ]. As it is well known, the main characteristic of phenolic compounds is

Open access

Black elder inflorescence has been traditionally used in Central Europe both in folk and official medicine. This plant material is a rich source of two biologically active components, rutin and chlorogenic acid. Nevertheless, there is a lack of data on the changes of their content during processing.The stability of rutin and chlorogenic acid during drying and the long-term storage of black elder inflorescence were analysed in this study. The rutin content was determined by capillary electrophoresis using solid-phase extraction. HPLC was used for the determination of chlorogenic acid. The dependence of rutin and chlorogenic acid content on the temperature of drying and storage duration were monitored and statistically evaluated by a two-way ANOVA test. The contents of rutin and chlorogenic acid revealed no statistically significant changes when dried at temperatures of 22 °C and 30 °C. The significant decrease in contents of both studied compounds was found at a drying temperature of 50 °C. The decrease in content of rutin was about 20%, in chlorogenic acid about 12%.The content of both studied compounds also decreased after long-term storage (at a temperature of 22 °C for one year). The decrease in content of rutin was greater than that of chlorogenic acid.

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The chlorogenic acid and the total polyphenol content were analysed in two different potato varieties (Kennebec, Agria) grown under five different nitrogen fertiliser rates (0, 75, 150, 225, 300 kg ha-1). Chlorogenic acid content ranged between 6.0-22.3 mg kg-1 fresh weight and was not influenced by fertiliser levels. The chlorogenic acid in potato tubers accounted for almost 90% of the total polyphenols. Free radical scavenging and antioxidant activities of the tubers were also analysed. Ethanolic extracts if the tubers showed marked hydrogen-donating activity in the experiment using 1,1-diphenyl-2-picrylhydrazyl (DPPH), they had reducing power as measured by the Fe(III)?Fe(II) reaction, but did not exhibit H2O2 scavenging activity assessed with a chemiluminescence method. Potato extracts showed significant, although weak Cu(II)-chelating activity and inhibited the autoxidation of linolenic acid as measured by the thiocyanate method. Chlorogenic acid containing extract of potato, can act as primary and secondary antioxidant in prevention of oxidative stress. The strong correlation between the antioxidant activity and the level of total pholyphenols suggests that the phenolic compounds are important antioxidant components of whole potato tubers. Variety had minimal, while nitrogen fertiliser rate had no effects on the levels of the studied characteristics.

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stress and increases gene expression of GRP78, IRE1, PERK, and ATF6 [ 13–15 ]. TM challenge is prevalently used to study ER stress pathways in cells and animals [ 13 , 15–17 ]. Chlorogenic acid (CA) is a phenolic compound of the Hydroxycinnamic acid

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High-performance thin-layer chromatography with densitometric detection has been used for quantitative analysis of chlorogenic acid in extracts obtained by different methods from the fruits of Peucedanum alsaticum . Separation was achieved on silica gel HPTLC plates with ethyl acetate-formic acid-acetic acid-water 100:11:11:21 as mobile phase. Densitometric detection was performed before and after derivatization with Naturstoffreagenz A at 320 nm. Calibration plots were linear in the range 10–75 μg mL −1 . The results obtained were very similar to those from HPLC, so this densitometric method can be easily used for easy and economic quantitative analysis of active compounds in plant material.

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Acta Chromatographica
Authors:
Mengya Lu
,
Qianqian Tang
,
Chenyu Zhou
,
Zhizheng Fang
,
Zheng Fan
,
Xiangyu Li
,
Rongchun Han
, and
Xiaohui Tong

batches collected from 12 pharmaceutical companies were collected and analyzed using six bioactive constituents (epigoitrin, caffeic acid, chlorogenic acid, acetylcorynoline, baicalin and baicalein) as the phytochemical markers. The results demonstrated

Open access

It was determined that amounts of chlorogenic acid, tyrosine, and tryptophan in potato tubers depend on meteorological conditions, farming type, individual properties of a variety, and interaction of these factors (P<0.05). It was noted in 2011 that organically grown potato tubers accumulated significantly higher amounts of chlorogenic acid, tyrosine, and tryptophan derivatives than conventionally grown tubers, while in 2012 the opposite was observed (P<0.01). Such results are due to the different meteorological conditions during the survey years.

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Ground ivy (Glechoma hederacea L.) is one of the prosperous plants for the food-industry as natural antioxidant. This fact led us to examine the chemical diversity of six ground ivy populations situated in different natural habitats and to analyse the effect of the harvesting time. Total phenolic content, chlorogenic acid, and rutin content, as well as the antioxidant capacity showed significant differences due to the harvest time. The highest total phenol content (115 mg g–1 GAE) and the strongest antioxidant activity (53.3 mg g–1 AAE) were measured in the population originated from Budapest (GLE 6), harvested in July. The highest chlorogenic acid (357 mg/100 g) and rutin (950 mg/100 g) contents were detected in the July harvested samples from the Soroksár Botanical Garden population (GLE 1). According to our results, the collection time has significant effect on the total phenolic content – first of all on the chlorogenic acid and rutin accumulation levels of ground ivy, while the influence of the habitat seems to be less important.

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for the quality control of SYD, which restricts the clinical application of this preparation. Recently, 14 compounds of SYD were identified in rat serum, of which 9 were in the original form, such as chlorogenic acid, isochlorogenic acid C

Open access
Acta Chromatographica
Authors:
Milica Atanacković Krstonošić
,
Jelena Cvejić Hogervorst
,
Mira Mikulić
, and
Ljiljana Gojković-Bukarica

. Experimental 2.1. Standards and Reagents Phenolic standards, namely, rutin, naringenin, chlorogenic acid, trans -cinnamic acid, quercetin, p -coumaric acid, caffeic acid, p -hydroxybenzoic acid, syringic acid, vanillic

Open access