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We present the comprehensive chromatographic profiles of three scorpion species, Androctonus crassicauda, Androctonus bicolor, and Leiurus quinquestriatus, commonly inhabited to Middle East regions. Their venoms were milked by electrical stimulation, diluted with distilled water, properly mixed and centrifuged to separate the mucus from venom. The clear supernatant was filtered and the protein concentration was determined. Pre-diluted venoms were chromatographed on FPLC (fast protein liquid chromatography) and RP-HPLC (reversed-phase high-performance liquid chromatography) and the fractions were collected for molecular weight determination. Both techniques have resulted clearly distinguishable chromatographic patterns that can be used for identification of scorpion species and having a quick indication of venom toxicity.

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The use of polypropylene materials in industry for food packaging is increasing. The presence of additives in the polymer matrix enables the modification or improvement of the properties and performance of the polymer, but these additives are potential risk for human health. In this context, an efficient analytical method for the quantitative determination of three antioxidants (2,6-di-tert-butyl-4-methylphenol (BHT), dibutylhydroxyphenylpropionic acid stearyl ester (Irganox 1076), and tns-(2.4-di-tert-butyl)-phosphite (Irgafos 168)) and five ultraviolet stabilizers (2-(2′-hydroxy-5′-methylphenyl) (UV-P), (2′-hydroxy-3′-tert-5′-methylphenyl)-5-chloroben zotriazole (UV-326), 2-(2′-hydroxy-3′,5′-di-tert-butylphenyl)-5-chlorobenzotriazole (UV-327), 2-(2H-benzotriazol- 2-yl)-4-(1,1,3,3-tetramethylbutyl)phenol(UV-329), and 2-hydroxy-4(octyloxy) benzophenone (UV-531)) in polypropylene food packaging and food simulants by high-performance liquid chromatography (HPLC) has been developed. Parameters affecting the efficiency in the process such as extraction and chromatographic condition were studied in order to determine operating conditions. The analytical method showed good linearity, presenting correlation coefficients (R ≥ 0.9977) for all additives. The limits of detection and quantification were between 0.03 and 0.30 μg mL−1 and between 0.10 and 1.00 μg mL−1 for eight analytes, respectively. Average spiked recoveries in blank polypropylene packaging and food simulants were in the range of 80.4–99.5% and 75.2–106.7%, with relative standard deviations in the range of 0.9–9.1% and 0.2–9.8%. Dissolving the polypropylene food packaging with toluene and precipitating by methanol was demonstrated more effective than ultrasonic extract with acetonitrile or dichloromethane for extracting the additives. The method was successfully applied to commercial polypropylene packaging determination, Irgafos 168 and UV-P were frequently found in six commercial polypropylene films, and the content ranged from 166.47 ± 5.11 to 845.27 ± 29.31 μg g−1 and 2.10 ± 0.29 to 19.23 ± 1.26 μg g−1, respectively.

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.D. Wilson (eds.), Recent advances in thin-layer chromatography, Plenum Press, London, 1988, pp. 45–54. Sticher O. Recent advances in thin

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Shahrzad, S. & Bitsch, I. (1996): Determination of some pharmacologically active phenolic acids in juices by highperformance liquid chromatography. J. Chromat. A. , 74 , 223–231. Bitsch I

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to the analytical laboratory, which performed the analysis of the concentration of pesticides. The performed analytical methods were consistent with US EPA 535 [ 15 ] and US EPA 1694 [ 16 ]. Concentration was determined by liquid chromatography using

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L.R. Snyder , in: Cs. Horvath (ed) High-performance Liquid Chromatography: Advances and Perspectives, Vol. 3., Academic Press, New York, 1983, pp. 157–223. Snyder L

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. Prosek, R.E. Kaiser , direct control of a CAMAG scanner to adjust plate position. R.E. Kaiser , Sorbfil video densitometer version 2

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, Chromatography of Proteins and Nucleic Acids, Science, Moscow, 1985. Osterman L.A. Chromatography of Proteins and Nucleic Acids 1985

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Journal of Thermal Analysis and Calorimetry
Authors: G. Fontanari, G. Souza, J. Batistuti, V. Neves, I. Pastre, and F. Fertonani


Glutelin, the major protein fraction from guava seed, was obtained by fractioning as described by Osborne. The total proteins were extracted and the isolates obtained by isoelectric precipitation presented similar DSC curves, concordant with the results obtained by gel filtration chromatography and electrophoresis in polyacrylamide gel (PAGE-SDS). However, the DSC curves showed a higher enthalpy with regard to the denaturing protein isolate (PI) extracted at pH 10.0 when compared to a PI at pH 11.5. Such results are in accordance with those obtained for PI extracted at pH 10.0 using chromatography, this one being present in the form of molecular aggregates of greater molecular mass. The glutelin fraction, however, did not present a denaturation peak in the DSC curve, showing that the process for obtaining the same significantly altered its conformation.

Restricted access N.A. Izmailov, M.S. Shreiber , Farmatsiya 3 (1938) 1

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