Authors:J.W Guo, Q. Li, W.Q. Chen, X. Li, L.Q. Li, T.G. Liu, Z.L. Ren, and P.G. Luo
Leaf senescence is a notably important trait that limits the yield and biomass accumulation of agronomic crops. Therefore, determining the chromosomal position of the expression sequence tags (ESTs) that are associated with leaf senescence is notably interesting in the manipulation of leaf senescence for crop improvement. A total of 32 ESTs that were previously identified during the delaying leaf senescence stage in the stay-green wheat cultivar CN17 were mapped to 42 chromosomes, a chloroplast, a mitochondrion, and a ribosome using in silico mapping. Then, we developed 19 pairs of primers based on these sequences and used them to determine the polymorphisms between the stay-green cultivars (CN12, CN17, and CN18) and the control cultivar MY11. Among the 19 pairs of primers, 5 pairs produced polymorphisms between the stay-green cultivar and the non-stay-green control. Further studies of Chinese Spring nullisomic-tetrasomics show that JK738991 is mapped to 3B, JK738983 is mapped to 5D, and JK738989 is mapped to 2A, 4A, and 3D. The other two ESTs, JK738994 and JK739003, were not assigned to a chromosome using the Chinese Spring nullisomic-tetrasomics, which indicates that these ESTs may be derived from rye DNA in the wide cross. In particular, the ESTs that produce polymorphisms are notably useful in identifying the stay-green cultivar using molecular marker-assisted selection. The results also suggest that the in silico mapping data, even from a comparison genomic analysis based on the homogeneous comparison, are useful at some points, but the data were not always reliable, which requires further investigation using experimental methods.
Authors:I. Leonova, E. Budashkina, K. Flath, A. Weidner, A. Börner, and M. Röder
A leaf rust resistance gene transferred from the tetraploid wheat
(Zhuk.) Zhuk. (genomic composition: A
GG) into common wheat
L. conditioned resistance at the seedling and adult plant stages in the introgression line ‘line 842-2’. To determine chromosome location and to map the resistance gene an F
population from a cross between ‘line 842-2’ and susceptible wheat cultivar ‘Skala’ was developed and screened against leaf rust pathotype 77 (
Erikss.). Microsatellite markers detected introgressions of the
genome on chromosomes 1A, 2A, 2B, 5B and 6B of ‘line 842-2’. Linkage analysis revealed an association between leaf rust resistance and microsatellite markers located on chromosome 5B. The markers
were closely linked to the resistance gene with genetic distances of 7.7 cM and 10.4 cM, respectively. Infection type tests with three leaf rust isolates resulted in different patterns of infection types of ‘line 842-2’ and ‘Thatcher’ near-isogenic line with the
gene on chromosome 5B. The data corroborated the hypothesis of the diversity of the resistance coming from
. The resistance gene of the introgression ‘line 842-2’ seems to be different than
and therefore it was designated
Adonina, I.G., Salina, E.A., Pestsova, E.G., Röder, M.S. 2005. Transferability of wheat microsatellites to diploid
species and determination of chromosomallocalizations of microsatellites in the S
Authors:Balázs Duga, Márta Czakó, Katalin Komlósi, Kinga Hadzsiev, Katalin Sümegi, Péter Kisfali, Márton Melegh, and Béla Melegh
., Raimond, E., Elgoyhen, A. B., et al.: Molecular cloning and chromosomallocalization of the human alpha7 nicotinic receptor subunit gene (CHRNA7). Genomics, 1994, 19 (2), 379–381.
Gault, J., Robinson, M., Berger, R
Chindamporn, A., Nakagawa, Y., Homma, M., Chibana, H., Doi, M., Tanaka, K.: Analysis of the chromosomallocalization of the repetitive sequences (RPSs) in Candida albicans . Microbiology 141 , 469 (1995).
Analysis of the chromosomal
Authors:A. F. Bálint, G. Kovács, A. Börner, G. Galiba, and J. Sutka
Galiba G., Kocsy, G., Kaursawhney, R., Sutka, J., Galston, A. W. (1993): Chromosomallocalization of osmotic and salt stress-induced differential alterations in polyamine content in wheat. Plant Science , 92 , 203