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wheat (Triticum aestivum) by chromosome elimination. Nature 256 :410–411. Barclay I.R. High frequencies of haploid production in wheat (Triticum aestivum) by chromosome elimination

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-London 82 : 29 – 33 . Chaudhary , H.K. 2008 . Dynamics of wheat × Imperata cylindrica – a new chromosome elimination mediated system for efficient haploid

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Chaudhary, H.K. 2008a. Dynamics of wheat × Imperata cylindrica — a new chromosome elimination mediated system for efficient haploid induction in wheat. In: Appels, R. et al. (eds), Proceedings of the 11th International Wheat Genetics Symposium. University of

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The relative efficiency of in vitro and in vivo screening techniques for drought tolerance, comprising various parameters, namely germination (%), shoot length, root length, coleoptile length, root number, root/shoot ratio and seedling vigour index (SVI) under in vitro conditions and morpho-physiological and yield-contributing traits under in vivo conditions, was studied using 78 winter × spring wheat-derived doubled haploid lines of bread wheat along with 13 parental genotypes and two check varieties, HPW 155 and PBW 343. Analysis of variance for different in vitro parameters in control (0 MPa) and stress (−0.7 MPa) environments and various in vivo parameters under irrigated and rainfed environments indicated sufficient genetic variability and the differential response of the genotypes to the different stress levels for all the in vitro and in vivo parameters. Correlation studies revealed the significance of percentage germination, root number, coleoptile length and seedling vigour index under in vitro conditions and relative water content and excised leaf water loss under in vivo conditions as important selection criteria for drought tolerance, as these parameters were related with each other as well as with the drought susceptibility index (S). The significant positive rank correlation between the in vitro (−0.7 MPa) and in vivo (rainfed) stress conditions indicated that the performance of a genotype under field conditions is very similar to its performance under laboratory conditions. Hence, the selection precision for a crucial and complex trait like drought tolerance in wheat can be enhanced by exercising in vitro selection coupled with evaluation in the field. The drought susceptibility index ‘S’ should not be taken as the sole criterion to categorize genotypes as drought-tolerant or susceptible ones.

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An investigation to standardize the protocol for in vitro application of colchicine for enhancing the doubled haploid production in wheat was done. Two tetraploid (PDW-314 and A-9-30-1); and two hexaploid (DH-40 and C-306) wheat genotypes were used as maternal parents, whereas, the pollen sources involved Zea mays (cv. Bajaura Makka) and Imperata cylindrica. During the rabi seasons of years 2013–14 and 2014–15, wheat × maize and wheat × I. cylindrica hybridization was carried out followed by treatment of their haploids produced as a result of elimination of chromosomes of maize and I. cylindrica respectively, with varied doses of colchicine for different durations The various doses of colchicine were categorized into two groups: lower doses for longer durations (0.01, 0.025, 0.05% each for 5, 7, 9, 11 hrs) and higher doses for shorter durations (0.05, 0.075, 0.10, 0.15, 0.20, 0.25% each for 5, 4, 3, 2 hrs). The response of different concentrations of colchicine applied for varied durations revealed significant differences for various doubled haploidy parameters viz., per cent survived plants, per cent doubled haploid formation and per cent doubled haploid seed formation. In hexaploid and tetraploid wheats, colchicine doses of 0.075% for 4 hrs and 0.15% for 4 hrs, respectively were established as optimum for enhanced doubled haploid production.

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.K. 2008 a. Dynamics of wheat × Imperata cylindrica –a new chromosome elimination mediated system for efficient haploid induction in wheat . In: Appels , R. Eastwood , R. , Lagudah , E. , Langridge , P. , Mackay , M. , McIntyre , L. , Sharp

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Chaudhary H.K. 2008. Dynamics of wheat Imperata cylindrica — Anew chromosome elimination mediated system for efficient haploid induction in wheat. In: Appels R., Eastwood R., Lagudah E., Langridge P., Mackay M., McIntyre L., Sharp P. (eds), Proc. 11th

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Gernand, D., Rutten, T., Varshney, A., Rubtsova, M., Prodanovic, S., Bruess, C., Kumlehn, J., Matzk, F., Houben A. 2005. Uniparental chromosome elimination at mitosis and interphase in wheat and pearl millet crosses involves micronucleus formation

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.R. 1975 . High frequencies of haploid production in wheat ( Triticum aestivum ) by chromosome elimination . Nature 256 : 410 – 111 . Campbell , A.W. , Griffin , W.B. , Conner , A

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., Mahn, A. (1994) Improved techniques for haploid production in wheat using chromosome elimination. Plant Breeding, 113: 125–129. Mahn A. Improved techniques for haploid production in

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