., Collis, K., Ramsden, M., Fox, H. M., Peberdy, J. F.: Chromosomerearrangement in improved cephalosporin C-producing strains of Acremonium chrysogenum . Curr Genet 19 , 235 (1991).
Chromosomerearrangement in improved
Authors:A. Sepsi, K. Németh, I. Molnár, É. Szakács, and M. Molnár-Láng
The aim of the experiments was to develop translocation lines by inducing homoeologous chromosome pairing in a 4H(4D) wheat-barley substitution line previously developed in Martonvásár. It was hoped to incorporate various segments of the barley 4H chromosome from the 4H(4D) substitution into wheat. Observations were made on the frequency with which wheat-barley translocations appeared in the F
progeny grains from a cross between the line CO4-1, which carries the
suppressor gene from
and thus induces a high level of homoeologous chromosome pairing, and the 4H(4D) wheat-barley substitution line, and on which chromosome segments were involved in the translocations. The translocations were identified by means of genomic
hybridisation. Of the 117 plants examined, three (2.4 %) were found to contain translocations. A total of four translocations were observed, as one plant contained two different translocations. The translocations consisted of one centric fusion, two dicentric translocations and one acrocentric chromosome. Plants carrying translocations were raised in the phytotron and the selection of homozygous translocation lines was commenced from the F
Authors:M. Naghavi, M. Ranjbar, A. Zali, M. Aghaei, M. Mardi, and S. Pirseyedi
Simple sequence repeat (SSR) DNA markers were used to characterize the genetic diversity in 70 accessions of
from Iran as well as to determine relationships among these accessions with 9 accessions of
) and 5
landraces. All twenty SSR primer pairs were polymorphic and identified a total number of 149 alleles corresponding to an average of 7.5 alleles per locus. The highest and lowest PIC values were obtained in subsp.
accessions, respectively. Data obtained were used to estimate genetic similarity using the Dice coefficient, and dendrogram was constructed using the UPGMA method. The dendrogram separated the 84 accessions into two main groups. All species grouped according to their genomes. A good level of genetic diversity was observed in the accessions of
, even in geographically close regions, which can be used in the broadening of the genetic base of bread wheat. In addition,
were clustered further away from
, confirming probably chromosomal rearrangements in the Dgenome of
during the processes of evolution.
Authors:George Filioussis, Ioannis Ioannou, Evanthia Petridou, Maria Avraam, Nektarios Giadinis, and Spyridon Kritas
A major concern with the use of tetracycline against mycoplasmas is the development of resistance. Infections in small ruminants due to tetracyclineresistant Mycoplasma agalactiae strains are becoming a frequent problem worldwide. In the present paper the detection and analysis of three tetracycline-resistant M. agalactiae strains, isolated from infected goats in Cyprus, are reported. The three field isolates were identified as M. agalactiae by polymerase chain reaction (PCR) showing 98% identity to the M. agalactiae PG2 reference strain. Furthermore, they were found sensitive to tylosin, enrofloxacin, spiramycin and lincomycin. In contrast, they were resistant to tetracycline. None of the putative genes [tet(M), tet(O) and tet(S)] that commonly contribute to high-level resistance to tetracycline could be amplified from their genome. Contrarily, the field isolates were found to carry ISMag1, an insertion sequence related to the IS30 family of mobile elements. Although ISMag1 is widely believed to induce high-frequency chromosomal rearrangements resulting in phenotypic changes of microorganisms, its potential role in tetracycline resistance of mycoplasmas requires further studies.
Authors:Pankaj B. Miniyar, Asha B. Thomas, Resham D. Kulkarni, Supriya A. Kadam, Parminder P. Chouhan, and Sohan S. Chitlange
Genetic mutations, chromosomal breaks, and chromosomal rearrangements, which are induced due to organic impurities, are considered as potential genotoxic impurities. The European Medicines Agency (EMA) and the United States Food and Drug Administration (US FDA) have set a threshold of toxicological concern (TTC) of 1.5 µg per person per day for each impurity. A sensitive and simple high-performance thin-layer chromatography (HPTLC) method has been developed and validated for determination of the potential genotoxic impurity, namely, 2-chloroaniline, at trace levels in quetiapine fumarate. The method was found to be specific and selective for the application. The limit of detection (LOD) and limit of quantification (LOQ) for quetiapine fumarate were found to be 1.27 and 3.87 ng per band. The LOD and LOQ values for 2-chloroaniline were found 0.018 and 0.054 ng per band, respectively. The calibration curve for 2-chloroaniline was linear over a concentration range from 2.5 to 12.5 ng. The method was found to be specific, precise, linear, and accurate and can be employed for monitoring and estimation of levels of 2-chloroaniline in quetiapine fumarate.