Authors:D. Duman, H. Akin, M. Deniz, G. Can, and B. Yegen
Bortolotti , M., Brunelli , F., Sarti , P., Mari , C. & Miglioli , M. (1998): Effects of oral clarithromycin and amoxicillin on interdigestive gastrointestinal motility of patients with functional dyspepsia and Helicobacter pylori gastritis. Aliment
Authors:B. Habibi, I. Ghorbel-Abid, R. Lahsini, D. Chehimi Ben Hassen, and M. Trabelsi-Ayadi
. Clarithromycin [6-O-methyl erythromycin] synthesized by substituting a methoxy group for the C-6 hydroxyl group of erythromycin (Figure 1c ) is a second-generation macrolide with broad spectrum of antibiotic activity [ 8 ]. It is active against the organisms
Clarithromycin used for the treatment of respiratory tract infection. Anew alternative simple, rapid, sensitive, and selective high-performance thin-layer chromatography (HPTLC) method was developed and validated for the estimation of clarithromycin in plasma. HPTLC analysis was performed on pre-coated silica gel 60F254 plates. Separation was carried out using ethyl acetate-methanol-15% ammonium acetate (pH 10.6) as solvent system. Densitometry measurements were performed in absorbance mode at 506 nm after derivatising with xanthydrol solution. The RF of the drug was found to be 0.62. The method was linear over the range of 0.1–3.0 μg mL−1 (r2 = 0.9974). The mean extraction recoveries were over 85%. The intra-day and inter-day precision (%CV) of the assay was in the range of 0.79–4.55%. The accuracy was found to be above 95%. The method was successfully applied to a pharmacokinetic study of clarithromycin 250 mg tablet in healthy human male volunteers.
The purpose of this work was to develop a TLC-densitometric method for simultaneous identification and quantitative determination of azithromycin, clarithromycin, roxithromycin, spiramycin, and troleandomycin. The method was developed on TLC aluminium plates precoated with silica gel F254 using solvent system izopropanol:n-hexane:ammonia 25% (8:12:3, v/v/v), which gives compact spots for azithromycin (RF = 0.65), clarithromycin (RF = 0.54), roxithromycin (RF = 0.49), spiramycin (RF = 0.22), and troleandomycin (RF = 0.36). Densitometric analysis was carried out at 478 nm after spraying with (1:4, v/v) sulphuric acid:ethanol and heating at 100°C for 5 min. The linear regression analysis data for the calibration plots showed good linear relationship with correlation coefficient higher than 0.99. The method is distinguished by high sensitivity, with limit of detection (LOD) from 0.34 μg/spot for troleandomycin to 0.67 μg/spot for clarithromycin and limit of quantification (LOQ) from 1.02 μg/spot for troleandomycin to 2.04 μg/spot for clarithromycin and a wide linearity range from 2 to 12 μg/spot for spiramycin and 2–15 μg/spot for other antibiotics. The precision of the determination was good; relative standard deviation (RSD) varied in the range from 1.49% to 4.14%.
Authors:Bahia A. Moussa, Ehab F. El-Kady, Marwa F. Mohamed, and Nadia F. Youssef
A selective densitometric analysis has been adopted for the determination of pantoprazole sodium sesquihydrate, metronidazole, and clarithromycin as they play an important rule as co-administrated drugs in the treatment of helicobacter infection. The aim of this work was to find greener thin-layer chromatographic (TLC) solvents to follow the environmental safety measurements. The three drugs are vastly different in chemical structure, so it was very challenging to carry out the simultaneous separation and quantitation by TLC technique at RF values 0.49 ± 0.01, 0.72 ± 0.01, and 0.83 ± 0.01, respectively. In the developed method, chromatography was performed on TLC plates with pre-coated silica gel 60 F254 using ethyl acetate and absolute ethanol (3:1)‒heptane‒ammonia 33% (14:5:1, v/v) as the developing system with calculated polarity (3.535). Densitometric measurements were carried out using CAMAG Linomat 5 TLC scanner at 280 nm. Regression line data were evaluated by the least square method within the concentration range of pantoprazole sodium sesquihydrate, metronidazole, and clarithromycin 0.8–8, 4–40, and 5–50 μg band−1, and the detection limits of the drugs were 0.15, 0.76, and 0.88 μg band−1, respectively. The suitability of this TLC method for the quantity determination of three compounds in drug substances and drug products was proved by validation in accordance with the International Conference on Harmonization (ICH) guidelines (Q2R1). Statistical analysis was performed using Student’s t and F test, revealing no significant difference between the TLC method and the official ones concerning accuracy and precision.
Authors:Isabel Stephany-Brassesco, Stefan Bereswill, Markus M. Heimesaat, and Matthias F. Melzig
-positive bacteria such as Bacillus cereus , Staphylococcus aureus and Enterococcus faecalis [ 16 ]. This prompted us to uncover potential synergistic antimicrobial effects of Cefabronchin ® and antibiotics such as amoxicillin and clarithromycin on clinical