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A study was conducted to compare the DNA structure of Streptococcus mutans strains in children with caries-active, caries-free, and gingivitis clinical diagnosis. Twenty-eight Streptococcus mutans strains from 100 children’s plaques were examined by pulsed-field gel electrophoresis (PFGE) method. The classified strains were closely related to one another, though the strains originated from different disease groups. Three identical pairs were found, but the pairs in two cases belonged to different disease groups.The results of the PFGE experiments suggest that there is no correlation between the different DNA patterns of S. mutans strains and their cariogenecity. So the different DNA strains of S. mutans are not the only determining factor in the development of dental caries.

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.: Autopsy and clinical diagnosis. Path. Res. Pract., 1980, 168 , 107–114. Drexler H. Autopsy and clinical diagnosis Path. Res. Pract

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Bevezetés: A spontán események monitorozásának kiterjesztése loop recorder beültetésével megnövelheti a definitív diagnózisok arányát a syncope kivizsgálása során. Célkitűzés: A szerzők retrospektív obszervációs vizsgálatban kívánták meghatározni az implantálható loop recorder diagnosztikus teljesítményét a syncope kivizsgálásában a mindennapi klinikai gyakorlat körülményei között. Módszer: A szerzők kardiológiai osztályán 2005 és 2014 közötti időszakban ismeretlen eredetű ismételt syncope miatt loop recorder beültetésen átesett összes beteg elektronikus adatbázisban tárolt adatait elemezték. Eredmények: A vizsgált időszakban 52 loop recorder beültetés történt. A monitorozás átlagosan 167 (±136) napja alatt 36 (69,2%) diagnosztikus esemény történt. Az események kétharmadában, azaz az összes vizsgálat 46,2%-ában a monitorozás arrhythmiás mechanizmust igazolt, amely ezen esetekben definitív terápiát tett lehetővé. Ebben a válogatott betegcsoportban az életkor, az ismert kockázatprediktorok és a syncopét kísérő trauma nem mutattak összefüggést az eszméletvesztés mechanizmusával. Következtetések: A loop recorder használatával a mindennapi gyakorlatban elért magas diagnózisarány összhangban van a klinikai vizsgálatok eredményeivel, ami alátámasztja a módszer korai alkalmazását a syncope kivizsgálási menetében. Orv. Hetil., 2015, 156(15), 609–613.

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. Results The study analytical sample included 869 MA residents with a clinical diagnosis of PG who sought care in the Commonwealth at some time during the study period. Overall, treatment-seeking patients who had a diagnosis of PG and co

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Orvosi Hetilap
Authors: Viktor Bencs, János Bencze, V. László Módis, Viktória Simon, János Kálmán and Tibor Hortobágyi

–2024. 29 Rizzo G, Arcuti S, Copetti M, et al. Accuracy of clinical diagnosis of dementia with Lewy bodies: a systematic review and meta-analysis. J Neurol Neurosurg Psychiatry 2018; 89: 358

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Interventional Medicine and Applied Science
Authors: Istvan Balint, Marta Nad, Adrienn Kiraly, Ottilia Bali, Adel Rashed and Laszlo Vizsy

We present an 11-year-old male child with an enormous appendix that was regarded as an appendiceal mucocele. The disorder is very rare and usually appears in middle aged patients. It is a clinical diagnosis. It could cause a variety of symptoms, especially, acute appendicitis and unidentified lesion in the right iliac fossa. According to the reasons, it could be just a curiosity without any relevancy or the sign of a malignant lesion with bad prognostic factors. The histopathological findings prove the origin.

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Acta Veterinaria Hungarica
Authors: Yakup Yildirim, Seval Bilge Dağalp, Volkan Yilmaz and Ali Faraji Majarashin

In this study, the physical examination of 22 cattle revealed clinical signs of malignant catarrhal fever (MCF). Peripheral blood leukocyte (PBL) samples of the 22 cattle, and nasal (n = 7) and conjunctival (n = 9) swab samples from 16 sheep from two different farms, were taken for laboratory examination. The clinical diagnosis of MCF in cows was confirmed by the detection of ovine herpesvirus type 2 (OvHV-2) DNA by polymerase chain reaction (PCR). OvHV-2 DNA was detected by nested-PCR in PBL of one cow with clinical signs and nasal (1/7)-conjunctival(1/9) swab samples of two sheep housed in the same barn. According to the sequence analysis, three slightly divergent viruses were detected. The results indicate the need for additional research in different regions of Turkey to gain a better understanding of the incidence of MCF and its implications for the livestock industry.

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Absztrakt

A laboratóriumi és képalkotó diagnosztika fejlődésével a nem kielégítő hatékonyságú, kellemetlen és fájdalmas rectalis digitális vizsgálat heveny vakbélgyulladás esetén nem szükségszerűen a fizikális vizsgálat része. Ugyanakkor egyes kérdéses esetekben hasznos. Ilyenkor azonban a megváltozott jogi és társadalmi környezet miatt a vizsgálat altatásban történő végzése mérlegelendő.

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Acta Microbiologica et Immunologica Hungarica
Authors: Erika Orosz, Nóra Szentmáry, Huba J. Kiss, Ágnes Farkas, István Kucsera and Zoltán Zsolt Nagy

Acanthamoeba has a worldwide distribution in the environment and it is capable of causing a painful sight-threatening disease of the cornea designated as Acanthamoeba keratitis (AK). Nowadays, the cases of AK have surged all over the world along with its disease burden due to increasing use of contact lenses used not only for optical correction but also for cosmetic purposes. In our present work, epithelial abrasion of a 27-year-old female soft contact lens wearer with keratitis was examined. Genotype identification was carried out with a real-time fluorescence resonance energy transfer polymerase chain reaction (PCR) assay based on sequence analysis of the 18S rRNA gene. Genotyping allowed the identification of a T8 group isolate. The analysis confirmed the importance of a complete diagnostic protocol, including a PCR assay, for the clinical diagnosis of AK from human samples. Acanthamoeba T8 should be considered as potential causative organism in keratitis in human.

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Simple real-time PCR assay with one set of primer and probe for rapid, sensitive qualitative and quantitative detection of Entamoeba histolytica has been used. Consensus sequences were used to amplify a species-specific region of the 16S rRNA gene, and fluorescence resonance energy transfer hybridization probes were used for detection in a LightCycler platform (Roche). The anchor probe sequence was designed to be a perfect match for the 16S rRNA gene of Entamoeba species, while the acceptor probe sequence was designed for Entamoeba histolytica, which allowed differentiation. The performed characteristics of the real-time PCR assay were compared with ELISA antigen and microscopical detection from 77 samples of individuals with suspected clinical diagnosis of imported E. histolytica infection. Stool and liver abscess pus samples were examined with analytical sensitivity of 5 parasites per PCR reaction. The melting curve means Tms (standard deviation) in clinical isolates were 54°C. The real-time assay was 100% sensitive and specific for differentiation of Entamoeba histolytica, compared with conventional ELISA or microscopy. This real-time PCR assay with melting curve analysis is rapid, and specific for the detection and differentiation of Entamoeba histolytica. The suitability for routine use of this assay in clinical diagnostic laboratories is discussed.

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