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the seasonal coronavirus HCoV-229E, in order to evaluate its universality and potential applicability against various human coronavirus strains. Materials and methods Viral particles and antibodies Human coronavirus 229E (HCoV-229E) was obtained from
Centers for Disease Control and Prevention: Severe respiratory illness associated with a novel coronavirus – Saudi Arabia and Qatar. 2012. MMWR Morb. Mortal. Wkly Rep., 2012, 61, 820. http
, & Potenza, 2019 ; Blycker, unpublished clinical observations). On January 25, 2020, Pornhub recorded the initial use of the search term “coronavirus,” and its past-30-day use as a search term, along with “corona” and “covid,” increased substantially
Feline enteric coronaviruses have three open reading frames (ORFs) in region 3 (3a, 3b, and 3c). All three ORFs were expressed with C-terminal eGFP and 3xFLAG tags in different cell lines and their localisation was determined. ORF 3a is predicted to contain DNA-binding and transcription activator domains, and it is localised in the nucleus and in the cytoplasm. ORF 3b is also predicted to contain DNA-binding and activator domains, and was found to localise in the mitochondrion. Besides that, in some of the non-infected and FIPV-infected cells nucleolar, perinuclear or nuclear membrane accumulation of the eGFP-tagged 3b was observed. The exact compartmental localisation of ORF 3c is yet to be determined. However, based on our co-localisation studies 3c does not seem to be localised in the ER-Golgi network, ERGIC or peroxisomes. The expression of 3c-eGFP is clearly cell type dependent, it is more stable in MARC 145 cells than in Fcwf-4 or CrFK cells, which might reflect in vivo stability differences of 3c in natural target cells (enterocytes vs. monocytes/macrophages).
Introduction The coronavirus disease 2019 (COVID-19) pandemic is an ongoing global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The outbreak of the coronavirus pandemic at the end of 2019 called for the rapid
(33.3%) had wheezes, while all 3 children with HCoV-OC43 infection had wheezes. Rhinorrhea was a major symptom (85.7–100%) in children infected with Coronavirus, Adenovirus and Enterovirus. Also, sore throat was a major symptom with most viruses. No
Introduction The first case of Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection was reported in India from Thrissur, Kerala, on January 30, 2020
The coronavirus (COVID-19) pandemic has significantly disrupted normal activities globally. Stay-at-home mandates and quarantines have increased consumption of digital entertainment, particularly online gaming and related activities (e.g., esports
References 1 Worldometer. COVID-19 Coronavirus Pandemic. Last update: March 27, 2020. Available from: https://www.worldometers.info/coronavirus/ [accessed: March 25
Introduction On March 11th, the World Health Organization declared the Coronavirus Disease 2019 (COVID-19) as a global pandemic [ 1 ]. Since the beginning of the pandemic, more than 373 million people have contracted
