the seasonal coronavirus HCoV-229E, in order to evaluate its universality and potential applicability against various human coronavirus strains. Materials and methods Viral particles and antibodies Human coronavirus 229E (HCoV-229E) was obtained from
, & Potenza, 2019 ; Blycker, unpublished clinical observations). On January 25, 2020, Pornhub recorded the initial use of the search term “coronavirus,” and its past-30-day use as a search term, along with “corona” and “covid,” increased substantially
Feline enteric coronaviruses have three open reading frames (ORFs) in region 3 (3a, 3b, and 3c). All three ORFs were expressed with C-terminal eGFP and 3xFLAG tags in different cell lines and their localisation was determined. ORF 3a is predicted to contain DNA-binding and transcription activator domains, and it is localised in the nucleus and in the cytoplasm. ORF 3b is also predicted to contain DNA-binding and activator domains, and was found to localise in the mitochondrion. Besides that, in some of the non-infected and FIPV-infected cells nucleolar, perinuclear or nuclear membrane accumulation of the eGFP-tagged 3b was observed. The exact compartmental localisation of ORF 3c is yet to be determined. However, based on our co-localisation studies 3c does not seem to be localised in the ER-Golgi network, ERGIC or peroxisomes. The expression of 3c-eGFP is clearly cell type dependent, it is more stable in MARC 145 cells than in Fcwf-4 or CrFK cells, which might reflect in vivo stability differences of 3c in natural target cells (enterocytes vs. monocytes/macrophages).
Introduction The coronavirus disease 2019 (COVID-19) pandemic is an ongoing global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The outbreak of the coronavirus pandemic at the end of 2019 called for the rapid
(33.3%) had wheezes, while all 3 children with HCoV-OC43 infection had wheezes. Rhinorrhea was a major symptom (85.7–100%) in children infected with Coronavirus, Adenovirus and Enterovirus. Also, sore throat was a major symptom with most viruses. No
The first case of Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection was reported in India from Thrissur, Kerala, on January 30, 2020
The coronavirus (COVID-19) pandemic has significantly disrupted normal activities globally. Stay-at-home mandates and quarantines have increased consumption of digital entertainment, particularly online gaming and related activities (e.g., esports
On March 11th, the World Health Organization declared the Coronavirus Disease 2019 (COVID-19) as a global pandemic [ 1 ]. Since the beginning of the pandemic, more than 373 million people have contracted