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Acta Microbiologica et Immunologica Hungarica
Authors: L. Kredics, Zsuzsanna Antal, A. Szekeres, L. Hatvani, L. Manczinger, Cs. Vágvölgyi, and Erzsébet Nagy

, E.: Production of extracellular proteases by human pathogenic Trichoderma longibrachiatum strains. Acta Microbiol Immunol Hung 52 , 223-229 (2004). Production of extracellular proteases by human pathogenic Trichoderma

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Acta Microbiologica et Immunologica Hungarica
Authors: L. Kredics, Zsuzsanna Antal, A. Szekeres, L. Manczinger, Ilona Dóczi, F. Kevei, and Elisabeth Nagy

Species belonging to the filamentous fungal genus Trichoderma are well known as potential candidates for the biological control of plant pathogenic fungi and as cellulase producers of biotechnological importance. Several data were published in the last decade also about the clinical importance of this genus, indicating that Trichoderma strains may be potential opportunistic pathogens in immunocompromised patients. However, there is a lack of information about the potential virulence factors of clinical Trichoderma strains. This study was designed to examine the extracellular proteolytic enzymes of six clinical T. longibrachiatum isolates. Supernatants from induced liquid cultures of the examined strains were screened for proteolytic enzyme activities with 11 different chromogenic p-nitroaniline substrates. The production of trypsin-like, chymotrypsin-like and chymoelastase-like protease activities cleaving N-Benzoyl-L-Phe-L-Val-L-Arg-p-nitroanilide, N-Succinyl-L-Ala-L-Ala-L-Pro-L-Phe-p-nitroanilide,and N-Succinyl-L- Ala-L-Ala-L-Pro-L-Leu-p-nitroanilide, respectively, was common among the strains examined. Separation of trypsin- and chymotrypsin-like activities by column chromatography revealed, that both systems are complex consisting of several isoenzymes. The pH-dependence of these two protease systems was also studied. Based on the results, the different isoenzymes seem to have different optimal pH values. Extracellular proteolytic enzymes may be involved in the pathogenecity of Trichoderma strains as facultative human pathogens.

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Acta Microbiologica et Immunologica Hungarica
Authors: L. Kredics, Zsuzsanna Antal, Ilona Dóczi, L. Manczinger, F. Kevei, and Elisabeth Nagy

Cefditoren is the active form of cefditoren pivoxil, a new, broad-spectrum oral cephalosporin with strong in vitro activity against penicillin-susceptible and resistant Streptococcus pneumoniae. In this study, the minimum inhibitory concentrations (MICs) of cefditoren were determined for a special selection of S. pneumoniae isolates known to be susceptible, moderately susceptible or fully resistant to penicillin; these isolates originated from the lower respiratory tract of adults with pneumonia or the upper respiratory tract of children with or without symptoms of infection. Some of this latter group of isolates exhibited extremely high MICs to penicillin (³32 mg/l), whereas the MICs of cefditoren did not exceed 2 mg/l. The MIC50 and MIC90 of cefditoren proved to be 0.25 and 1.0 mg/l, respectively, with a range of MICs £0.015-2.0 mg/l for all the tested S. pneumoniae isolates. Its good activity suggests that cefditoren is expected to be a potent drug in infections caused by penicillin-resistant and multidrug-resistant S. pneumoniae.

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1435 1436 William, C. D., Tonic, P. C., Stratrian, C., Leeman, U., Harmon, S.: Isolation and properties of extracellular protease of Trichophyton granulosum . Biochem et

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320 vanKuyk, P. A., Cheetham, B. F., Katz, M. E.: Analysis of two Aspergillus nidulans genes encoding extracellular proteases. Fungal Genet Biol 29 , 201–210 (2000

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402 Benito, M. J., Rodriguez, M., Nunez, F., Asensio, M. A., Bermudez, M. E., Cordoba, J. J.: Purification and characterization of an extracellular protease from Penicillium chrysogenum

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., 32 , 1487 – 1495 . N ASCIMENTO , W.C. A & M ARTINS , M.L.L. ( 2004 ): Production and properties of an extracellular protease from thermophilic Bacillus sp . Braz. J

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6 129 139 Venugopal, V., Alur, M. D., Lewis, N. F.: Extracellular protease from Pseudomonas marinoglutinosa: Some properties and its action on

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Acta Biologica Hungarica
Authors: L. Kredics, Kata Terecskei, Zsuzsanna Antal, A. Szekeres, L. Hatvani, L. Manczinger, and Cs. Vágvölgyi

Kredics, L., Antal, Z., Szekeres, A., Hatvani, L., Manczinger, L., Vágvölgyi, C., Nagy, E. (2005) Extracellular proteases of Trichoderma species — a review. Acta Microbiol. Immunol. Hung. 52 , 169

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. Extracellular protease and phosholipase C are controlled by the global regulatory gene gacA in the biocontrol strain Pseudomonas fluorescens CHA0 FEMS Microbiol. Lett. 1994

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