The present paper reports a validated high-performance thin-layer chromatography (HPTLC)‒densitometric method for the simultaneous quantification of phenolic (ferulic acid and caffeic acid) and terpenoid (β-sitosterol and lupeol) markers in Convolvulus pluricaulis Choisy. According to Ayurveda, it is commonly known as ‘Shankhpushpi’ due to its ‘Conch’ or ‘Shankh’-shape flower. The plant species, viz., Clitoria ternatea L., Evolvulus alsinoides (L.) L., and Tephrosia purpurea (L.) Pers., also having similar flowers are reported as its adulterants/substitutes. This creates a problem in its quality and efficacy in the commercial drug market of India. Therefore, a HPTLCmethod was performed on a pre-coated silica gel 60 F254 plate with the aforesaid markers. The solvent system toluene–ethyl acetate–formic acid (8.5:1.5:0.1) was determined to be the best system for the simultaneous separation of caffeic acid, ferulic acid, β-sitosterol, and lupeol at RF values of 0.14, 0.29, 0.48, and 0.63, respectively. A densitometric scanning profile of all the samples at 580 nm showed peaks for all the four markers of varying heights in the samples, except the absence of caffeic acid in Tephrosia purpurea. The developed method was standardized and validated for the quantification of active principal-based quality-control markers in terms of precision, accuracy, linearity, recovery, and repeatability. It will help to maintain batch-to-batch consistency and identification of adulterants/substitutes in raw materials during production of drug in the pharmaceutical units.
Authors:Liang Zheng, Mengyue Wang, Zhong Chen, Jincai Hou and Xiaobo Li
cinnamic acid) [ 15 – 17 ]. On the other hand, the assistant herb, Angelicae sinensis radix, supported the monarch and minister herbs to reach target positions and exert their effects. It mainly consists of organic acids (such as ferulicacid) and volatile
Authors:Shasha Tang, Xiaoying Zhou, Haonan Yu and Yun Sun
High-performance thin-layer chromatography, which is a quick qualitative and quantitative method, was used to determine 3 kinds of organic acids, namely, ellagic acid, gallic acid, and ferulic acid, in Elaeagnus angustifolia L. leaves originating from 10 regions in Xinjiang Uygur Autonomous Region and 7 different growth stages in Urumqi, China. The developing solvent was toluene‒ethyl formate‒formic acid (5:6.55:1.49), the temperature was 25 ± 1°C, the humidity was 10%, and the detection wavelength was 290 nm. The linear ranges of ellagic acid, gallic acid, and ferulic acid were 0.0564–0.6768, 0.1006–1.2072, and 0.1004–1.2048 μg, respectively, and the linear correlation coefficients were 0.9953, 0.9993, and 0.9970, respectively. The results showed good linear relationships. Precision analyses revealed a relative standard deviation (RSD) of <2.00%. The sample was stable within 24 h at room temperature at RSD ≤3.00%. The average recoveries of ellagic acid, gallic acid, and ferulic acid were 101.4%, 100.9%, and 100.2%, respectively. This method is reliable and can be used to determine three organic acids in the leaves of E. angustifolia L. from different regions and different growth stages.
Authors:Urszula Hubicka, Jan Krzek, Jolanta Kaleta and Aneta Niedźwiedź
An HPTLC-densitometric method has been developed for identification and quantitative analysis of caffeic,
-coumaric, and ferulic acids in propolis concentrates. Solid-phase extraction on a column packed with a quaternary amine adsorbent was used for preliminary purification of concentrates. The compounds were successfully separated on silica gel plates with dichloromethane-acetonitrile-90% formic acid, 9.5 + 0.5 + 0.1 (
), as mobile phase. Densitometric analysis was performed in the UV at
= 320 nm. Under these conditions the method was highly sensitive and precision and comparability of results were good, as was proven by method validation and statistical analysis of the results obtained. Limits of detection were 29.50, 8.32, and 17.20 ng for caffeic,
-coumaric, and ferulic acids, respectively. The corresponding recoveries were 99.99, 99.88, and 99.89%. The linearity ranges were from 2.7 to 10.7 μg mL
, 2.1 to 17.6 μg mL
, and 2.2 to 18.3 μg mL
-coumaric, and ferulic acids, respectively. The precision of the method, expressed as
, was within the range 2.21 to 4.47%.