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): Production of the fructo-oligosaccharides by levansucrase from Bacillus subtilis C 4 . Process Biochem., 32 , 237 – 243 . Gonçalves , B.C.M. , Mantovan

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170 175 Bornet, F.R.J., Brouns, F., Tashiro, Y. & Duvillier, V. (2002): Nutritional aspects of short-chain fructooligosaccharides: natural occurrence, chemistry

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bulbs and its key role in the synthesis of fructo-oligosaccharides in vivo . New Phytol. , 165 , 513–524. Shiomi N. Purification and characterization of a

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Benkeblia, N. & Shiomi, N. (2006): Hydrolysis kinetic parameters of DP 6, 7, 8, and 9–12 fructooligosaccharides (FOS) of onion bulb tissues. Effect of temperature and storage time. J. agric. Fd

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BORNET, F.R.J., BROUNS, F., TASHIRO, Y. & DUVILLIER, V. (2002): Nutritional aspects of short-chain fructooligosaccharides: natural occurrence, chemistry, physiology and health implications. Digestive Liv. Dis

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Acta Alimentaria
Authors: M. Bekers, M. Marauska, M. Grube, D. Karklina, and M. Duma

Yun, J. (1996): Fructooligosaccharides - occurrence, preparation and application. Enzyme Microbiol. Technol , 19 , 107-117. Fructooligosaccharides - occurrence, preparation and

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production of fructooligosaccharides. Asian J. Microbiol. Biotechnol. Environ. Sci. , 5 , 313–318. Prapulla S.G. Microbial production of fructooligosaccharides

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Introduction Fructooligosaccharides (FOS) are considered to be physiologically favorable food ingredients that may improve the balance of intestinal microflora ( Sangeetha et al., 2005 ). A wide variety of health benefits has been claimed in

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JPC - Journal of Planar Chromatography - Modern TLC
Authors: Katarína Reiffová, Jana Podolonovičová, Andrej Oriňák, Karol Flórián, and Tat’ána Gondová

A rapid, simple qualitative thin-layer chromatographic method has been developed for separation of mono- and fructooligosaccharides from feed additives with the aim of analyzing them in biological materials. The fructooligosaccharides were analyzed in the commercially available dietetic feeding products Raftifeed and Raftilose (Orafti, Tienen, Belgium) and in the polysaccharide maltodextrin. Silica gel layers were pretreated either with sodium acetate or with a mixture of chloroform and methanol, and three different mobile phases were used. High-performance amino-bonded layers were developed with acetonitrile-water-phosphate buffer without previous pretreatment of the layers. Post-chromatographic derivatization of chromatograms was accomplished with dipheny-lamine-aniline-phosphoric acid reagent. Densitometric evaluation of chromatograms was performed at λ = 370 nm.

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A first attempt at the separation of fructo-oligosaccharides (FOS) and inulin mixtures is presented. Preliminary results obtained by automated multiple development (AMD) of diol layers with an acetonitrile-acetone-water polarity gradient are reported. The method is very promising and highly suitable for simple, direct analysis of complex mixtures of fructo-oligosaccharides (FOS) and inulin in samples of natural origin.

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