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In this study, synchronous spontaneous, independent liver and gallbladder tumours were detected in a Bearded dragon (Pogona vitticeps). The multiple tumours consisted of intrahepatic cholangiocarcinoma as well as in situ adenocarcinoma and two adenomas of the gallbladder. The biliary epithelial cells and the cholangiocarcinoma showed membranous cross-immunoreactivity for claudin-7. The gallbladder epithelial cells, its adenoma and adenocarcinoma showed basolateral cross-reactivity for claudin-7. We think that the humanised anti-claudin-7 antibody is a good marker for the detection of different primary cholangiocellular and gallbladder tumours in Bearded dragons. The cholangiocytes, the cholangiocarcinoma, the endothelial cells of the liver and the epithelial cells and gallbladder tumours all showed claudin-5 cross-reactivity. The humanised anti-cytokeratin AE1–AE3 antibody showed cross-reactivity in the biliary epithelial cells, cholangiocarcinoma cells, epithelial cells and tumour cells of the gallbladder. It seems that this humanised antibody is a useful epithelial marker for the different neoplastic lesions of epithelial cells in reptiles. The humanised anti-α-smooth muscle actin (α-SMA) antibody showed intense cross-reactivity in the smooth muscle cells of the hepatic vessels and in the muscle layer of the gallbladder. The portal myofibroblasts, the endothelial cells of the sinusoids and the stromal cells of the cholangiocarcinoma and gallbladder tumours were positive for α-SMA. The antibovine anti-vimentin and humanised anti-Ki-67 antibodies did not show crossreactivity in the different samples from the Bearded dragon.

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Acta Veterinaria Hungarica
Authors: Roland Psáder, Ágnes Sterczer, Kinga Pápa, Andrea Harnos, Viktória Szilvási, and Ákos Pap

Conter, R. L., Roslyn, J. J. and Taylor, I. L. (1987 a ): Effects of peptide YY on gallbladder motility. Am. J. Physiol. 252 , G736–G741. Taylor I. L. Effects of peptide YY on

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Acta Veterinaria Hungarica
Authors: Ágnes Sterczer, Jenő Reiczigel, Roland Psáder, Kinga Pápa, Károly Vörös, and Ákos Pap

Boer, S. Y., Masclee, A. M., Jebbink, M. C. W., Schipper, J., Jansen, J. B. and Lamers, C. B. (1992): Effect of intravenous fat on cholecystokinin secretion and gallbladder motility in man. J. Parent. Enter. Nutr

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Acta Veterinaria Hungarica
Authors: K. Vörös, T. Németh, T. Vrabély, F. Manczur, J. Tóth, Melinda Magdus, and Edina Perge

. Brömel , C. , Smeak , D. D. and Léveillé , R. ( 1998 a ): Porcelain gallbladder associated with primary biliary adenocarcinoma in a dog . J. Am. Vet. Med. Assoc. 213 , 1137 – 1139

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Washizu, T., Ikenaga, H., Washizu, M., Ishida, T., Tomoda, I. and Kaneko, J. J. 1990: Bile acid composition of dog and cat gall-bladder bile. Nippon Juigaku Zasshi. 52 , 423-425. Bile

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2839 2845 Behar, J., Corazziari, E., Guelrud, M., et al.: Functional gallbladder and sphincter of Oddi disorders. Gastroenterology, 2006, 130 , 1498

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Abdel Rahman, M., Blázovics A., Ágoston M. és mtsai: A chemiluminescent study for detection of free radicals in gallbladder bile in gallstone diseases. Ces. a Slov Gastroent., 1995, 49 , 7–13. Ágoston M

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We present a case of a middle-aged female who attended for a routine laparoscopic cholecystectomy as a day case surgery. At operation, she was found to have a distended gallbladder with an unusually prominent distal portion. This has made the dissection of the Calot’s triangle challenging. As a result, the “critical view of safety” technique was applied. This allowed for the clear Calot’s triangle visualization and identification of the cystic duct and artery. This case highlights that the knowledge of various ways of the cystic duct dissection is essential to every surgeon. Furthermore, this helps to adjust the dissection approach on an individual case bases ensuring avoidance of the common bile duct injuries.

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This paper is the first report on the molecular characterisation of myxozoan parasites from the odontobutid fish Chinese (Amur) sleeper (Perccottus glenii Dybowski, 1877). The authors determined the partial 18S rDNA sequence of Myxidium shedkoae Sokolov, 2013 from the gallbladder of the fish. Phylogenies reconstructed using maximum likelihood and Bayesian inference analysis revealed that M. shedkoae belongs to the hepatic biliary group of myxozoans (after Kristmundsson and Freeman, 2013) as a member of the clade consisting of Zschokkella sp. KLT-2014, Myxidium truttae and Zschokkella nova. Some new morphological features of the parasite are also presented.

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Orvosi Hetilap
Authors: Călin Molnar, Tibor Sárközi, Cedric Kwizera, Marian Botoncea, Opriș Zeno, Marius Petrișor, and Bianca Liana Grigorescu

References 1 Moon AM, Hamilton Howe J, McGinty KA, et al. Gallbladder agenesis mimicking cholelithiasis in an adult. Radiol Case Rep. 2018; 13: 640

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