Authors:Yang Zhi, Zhang Meiying, Lin Baohe, Han Yan, Mu Aping, Zhang Qing and Xu Xiaobao
Monoclonal antibody 3H11 was labelled with99mTc by modified Schwartz method. The antibody was incubated in a glass test tube at room temperature for 30min with a 1000-fold molar excess of 2-mercaptoethanol. The mean labelling efficiency of 3H11 with99mTc was more than 95%. The immuonreactivity of99mTc-3H11 was more than 80% by ELISA's method. Competetion results in vitro and HPLC analysis showed that99mTc was combined at the high affinity sites of antibody. The biodistribution in nude mice bearing 823 gastric cancer xenograpfts showed that the radioactivity in tumor at 24h postinjection was the highest except for that in kidney. The tumor uptake was 8.98±2.42% i.d/g. The ratio of tumor to blood was over 1.5 and that of tumor to liver was more than 2.5 at 24h post-injection. The tumor was clearly imaged at 22h postinjection. The inital clinical results showed that99mTc-3H11 was stable in vivo and was good located at the lesion sites.
Authors:Ning Liu, Jiannan Jin, Shuyuan Zhang, Shangwu Mo, Yuanyou Yang, Juan Wang and Maolun Zhou
An antigastric cancer monoclonal antibody, 3H11 and its Fab fragment, were labeled with #-emitter 211At using p-[211At] astatobenzoic acid (PAtBA) intermediate. The astatinated antibodies had conspicuous cytotoxic effect on human gastric cancer cell M85 in vitro. Tissue distribution of the astatinated antibodies were investigated in nude mice with subcutaneous tumor xenografts by i.v. injection. The astatinated Fab fragment was better suitable for 7.2-hour half life of 211At, since its tumor uptake remained higher (9.48–8.42 I.D%/g) than the astatinated intact antibody (~4.0 I.D%/g) from 3 to 14-hour post injection. However, the undesired high 211At uptake of the astatinated antibodies in some normal tissues, such as stomach, kidney and lung, suggested that the 211At labeled antibodies should be further explored.
Authors:László Czakó, Zoltán Szepes and Attila Szepes
. W., Lee, D. H., Lee, S. H., et al.: Preoperative staging of gastriccancer by endoscopic ultrasonography and multidetector-row computed tomography. J. Gastroenterol. Hepatol., 2010, 25 , 512–518.
Lee S. H