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Acta Agronomica Hungarica
Authors: M. Hudcovicová, V. Šudyová, S. Šliková, E. Gregová, J. Kraic, F. Ordon, D. Mihálik, V. Horevaj, and Z. Šramková

Marker-assisted selection (MAS) is an efficient modern method for transferring alleles or specific chromosome segments including important agronomic traits into elite cultivars. This approach makes genotypic selection possible, whereby the selection process is more effective. The Research Institute of Plant Production Piešťany uses genetic markers linked to important traits in the following pre-breeding programmes: 1. development of winter barley lines resistant to BaYMV/BaMMV, 2. development of spring barley lines resistant to BYDV, 3. development of winter wheat lines resistant to leaf rust (gene pyramiding), 4. improvement of wheat quality by new combination(s) of known HMW-GS and/or by introduction of novel HMW-GS alleles. Several hundreds of genotypes are usually analysed for the presence or absence of linked molecular markers and selected for use in breeding programmes.

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., Gazza, L., Pasquini, M. 2008. Resistance gene pyramiding in common wheat as a strategy to control rust diseases. In: Proc. 18th Eucarpia General Congress: Modern Variety Breeding for Present and Future Needs. Valencia, Spain, 9–12 September 2008, pp. 445

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. Evidence for enhanced resistance to diverse isolates of pearl millet downy mildew through gene pyramiding. Int. Sorghum and Millets Newsl. 47 :134–138. Bhaskar Raj G. Evidence for

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. pp. 4–6. Joshi, R. K., Nayak, S. (2010): Gene pyramiding — A broad spectrum technique for developing durable stress resistance in crops. Biotechnology and Molecular Biology Reviews , 5(3) , 51

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