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Cereal Research Communications
Authors: I. Baracskai, G. Balázs, L. Liu, W. Ma, M. Oszvald, M. Newberry, S. Tömösközi, L. Láng, Z. Bedő, and F. Békés

182 Bedõ, Z., Vida, Gy., Láng, L., Juhász, A., Karsai, I. 1999. Breeding a wheat variety with different lines for technological quality and HMW glutenin composition. J. Genet. Breed. 53 :57

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., Békés, F. 2011. A retrospective analysis of HMW and LMW glutenin alleles of cultivars bred in Martonvásár, Hungary. Cereal Res. Commun. 39 :226–237. Békés F. A retrospective analysis

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Anwar, R., Masood, S., Khan, M.A., Nasim, S. 2003. The high-molecular-weight glutenin subunit composition of wheat ( Triticum aestivum L.) landraces from Pakistan. Pak. J. Bot. 35 :61

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79 279 285 Anjum, F.M., Khan, M.R., Din, A., Saeed, M., Pasha, I., Arshad, M.U. 2007. Wheat gluten: High molecular weight glutenin subunits

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Glutenin and gliadin subunits play a key role in flour processing quality by network formation in dough. Wild relatives of crops have served as a pool of genetic variation for decades. In this study, 180 accessions from 12 domesticated and wild relatives of wheat were characterized for the glutenin and gliadin genes with allele-specific molecular markers. A total of 24 alleles were detected for the Glu-A3 and Gli-2A loci, which out of 19 amplified products identified as new alleles. Analysis of molecular variance (AMOVA) indicated that 90 and 65% of the genetic diversity were partitioned within two Aegilops and Triticum genera and their species, respectively. Furthermore, all glutenin and gliadin analyzed loci were polymorphic, indicating large genetic diversity within and between the wild species. Our results revealed that allelic variation of Glu-3A and Gli-As.2 is linked to genomic constitutions so that, Ae. caudata (C genome), Ae. neglecta (UM genome), Ae. umbellulata (U genome) and T. urartu (Au genome) harbor wide variation in the studied subunits. Hence, these species can be used in wheat quality breeding programs.

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Plant sucking aphids cause both quantitative and qualitative yield losses in cereals; moreover aphid-transmitted viruses are responsible for other quantitative and qualitative damages, thus direct or indirect effects of aphid infection are in focus of interest. Bread-making quality of wheat flour is determined primarily by the protein content and composition, the gluten proteins (glutenins, gliadins) being the prime factors. Allelic composition of the gliadin- and glutenin loci as well as the absolute amount and/or the relative ratio of gliadins to glutenins are very important in dough making and in determining baking quality. Wheat plants were caged at the beginning of stem elongation. Cages were treated with 0.1% methyl parathion. One week later, the caged plants were artificially infected with 5 alata individuals of Metopolophium dirhodum, Diuraphis noxia, Sitobion avenae and Rhopalosiphum padi. Flour from grains originating from plants infected artificially with cereal aphids were analyzed for glutenin and gliadin and total protein content, using Size Exclusion HPLC. It was found that aphid infection had significant effect on the glutenin and gliadin content, the total protein content and the gliadin/glutenin ratio. Both the glutenin and gliadin content was significantly higher in the seeds harvested from aphid infected plants. However, the gliadin/glutenin ratio was significantly lower in wheat flour prepared from aphid infected plants than in those from uninfected control. The most significant decrease in gliadin/glutenin ratio was caused by M. dirhodum, D. noxia, S. avenae infection followed by R. padi at high-abundance and low-abundance, respectively. As the gliadin/glutenin ratio was significantly lower in flours made from aphid infected wheat seeds, it may be suggested that aphid feeding results in decreased bread making quality of wheat flour.

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Cereal Research Communications
Authors: D. Horvat, N. Ðukić, D. Magdić, J. Mastilović, G. Šimić, A. Torbica, and D. Živančev

Anderson, O.D., Bekes, F., D’Ovidio, R. 2011. Effects of specific domains of high-molecular-weight glutenin subunits on dough properties by an in vitro assay. J. Cereal Sci. 54 :280

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of dough strength and end-use quality in durum wheat. In: Wrigley, C., Bekes, F., Bushuk, W. (eds), Gliadin and Glutenin — The Unique Balance of Wheat Quality. Am. Assoc. of Cereal Chemists, St. Paul, MN, USA, pp. 281

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Bietz, J.A., Shepherd, K.W., Wall, J.S. 1975. Single-kernel analysis of glutenin: use in wheat genetics and breeding. Cereal Chem. 52 :513–532. Wall J

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Atanasova, D., Tsenov, N., Todorov, I., Ivanova, I. 2009. Glutenin composition of winter wheat varieties bred in Dobrudzha agricultural institute. Bulgarian Journal of Agricultural Science 15 : 9

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