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Acta Veterinaria Hungarica
Authors: Paola Scarpa, Beatrice Ruggerone, Sara Gironi, Tiziana Vitiello, and Saverio Paltrinieri

Institutional Ethical Committee) a formal approval of the Institutional Ethical Committee was not necessary. Laboratory analyses Haematology was performed using the laser-based ADVIA 120 haematology system (Siemens Healthcare Global, Erlangen, Germany

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Haematological and biochemical analyses of blood were performed in carp (Cyprinus carpio L.) kept in small ponds. Caught and anaesthetised carp were clinically examined and blood samples were taken at regular intervals during the three years. In the first year of examinations, the haemoglobin and haematocrit values of carp fry significantly increased (P<0.01) from June to September. The intensive growth of carp in the summer period in the second year was accompanied by adequate erythropoiesis. During hibernation haematocrit and haemoglobin significantly decreased (P<0.05) and mean corpuscular haemoglobin concentration (MCHC) increased (P<0.01) in both scaly and mirror carp. MCHC increased also with the age and increasing body weight of the fish. Mirror carp had lower haematocrit and haemoglobin values than scaly carp (P<0.01). Comparative haematological analyses between carp of normal and poor body condition showed that moderate anaemia appeared in those with poor body condition. The results indicate that there is marked seasonal and age-dependent variation in the values of haematocrit and haemoglobin. Pond water quality investigations indicated good environmental conditions. A 50% increase (P<0.05) of glucose concentration was found from June to September in the blood plasma of carp in the third year, accompanied by an even more increased (80%; P<0.01) concentration of total lipids. At the same time, considerable changes of cholesterol and total protein concentrations were not observed. The results suggest that the investigated haematological and biochemical variables could be successfully utilised in monitoring the metabolic balance and health status of fish in intensive culture.

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Acta Veterinaria Hungarica
Authors: Vasile-Ioan Muntean, Orsolya Sárpataki, Adrian-Valentin Potârniche, Hemza Meghzili, Bogdan Sevastre, and Ioan Marcus

. , Campbell , T. W. , DeNicola , D. , Fettman , M. J. , Lassen , D. E. , Rebar , A. and Weiser , G. (eds) Veterinary Hematology and Clinical Chemistry . Lippincott Williams & Wilkins , Philadelphia . pp. 197 – 211

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103 110 Averbeck, C. (1992) Haematology and blood chemistry of healthy and clinically abnormal great black-backed Gulls (Larus marinus) and herring gulls (Larus argentatus

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Acta Microbiologica et Immunologica Hungarica
Authors: Ágnes Dencs, Ágnes Farkas, Mónika Gyugos, Andrea Kurcz, Erzsébet Puskás, B. Tresó, Erzsébet Rusvai, Erzsébet Barcsay, and Mária Takács

A nosocomial Hepatitis B virus (HBV) outbreak at a paediatric onco-haematology unit was investigated using molecular biological methods to determine the origin of the infections. The National Reference Laboratory of Hepatitis Viruses received seven HBsAg positive sera from patients and one from the brother of a patient. A fragment of the preS1/preS2/S genes from all samples was amplified, the PCR products were sequenced and a rooted phylogenetic tree was constructed. All nucleotide sequences from the different patients were very similar and 6 of the 8 sequences were identical, suggesting a common origin of the infections. These sequences were closely related to those amplified from a nosocomial HBV epidemic in another hospital in Hungary. The on-scene investigation revealed several malpractices. The two hospital departments had close connections and some of the patients were treated in both institutions. Present report underlines the importance of developing screening protocols for hepatitis viruses and that of the introduction of regular training programs for health care professionals in the field of hospital hygiene.

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Labbe, R. F. and Rettmer, R. L. (1989): Zinc protoporphyrin: A product of iron-deficient erythropoiesis. Seminars in Hematology 26 , 40--46. Zinc protoporphyrin: A product of iron-deficient erythropoiesis

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, Ullum H , Zacho M : Exercise-induced immunomodulation: possible roles of neuroendocrine factors and metabolic factors . Int. J. Sports Med . 18 , S2 – S7 ( 1997 ) 30. Pilny AA : Clinical hematology of rodent species. Vet. Clin. Exot

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Abstract

Infections caused by methicillin-resistant Staphylococcus aureus (MRSA) are responsible for rising health care costs and have a high attribution to mortality. Reliable and rapid detection of MRSA carriage is essential. Real-time PCR allows an early detection of MRSA colonization within 2 h. By using the BD GeneOhm-MRSA assay we analysed directly swabs of different sampling sites and compared the assay with culture method. One thousand one hundred and sixty samples from 129 patients in Magdeburg were examined. Of the samples, 8 (0.69%) or 1117 (96.3%) were tested equally positive or negative by both methods whereas 16 (1.38%) specimens were MRSA positive only by the GeneOhm-MRSA assay and 6 (0.52%) were MRSA positive only by culture method. Thirteen samples (1.12%), which are culture negative, were unresolved by the GeneOhm-MRSA. With regard to the patients, seven were detected as MRSA carriers only by the GeneOhm-MRSA while one patient was tested positive for MRSA only by culture. Assuming 100% correct results by the culture method, sensitivity and specificity of GeneOhm-MRSA assay could be calculated as 84.4% and 96.1% for nasal swabs, 78.7% and 96.9% for all swabs under study, and 94.8% and 99.5% when focussed on patients. PPV and NPV were 70.3% and 98% for all specimens together, respectively. BD GeneOhm-MRSA assay is a sensitive test for the detection of MRSA colonization from swab specimens without the need for an initial culture, but should always be performed in parallel to the culture method for comparison reasons. Furthermore, our results indicate that in addition swabs taken from different body sites were successfully analysed by the BD GeneOhm-MRSA assay. However, we conclude that the PCR assay might not be a preferred tool for screening in haematologic patients with low MRSA rate; for screening haematologic patients, the culture method is sufficient enough.

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